NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	K8B88_RS22160	Genome accession	NZ_CP082774
Coordinates	4524555..4525679 (-)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	A0A0H3EME5
Organism	Escherichia coli O16:H6 strain CU37RT-2
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 4519555..4530679

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8B88_RS22130 (K8B88_22045)	yrdA	4521355..4521909 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
K8B88_RS22135 (K8B88_22050)	yrdB	4521885..4522142 (-)	258	WP_001070567.1	DUF1488 domain-containing protein	-
K8B88_RS22140 (K8B88_22055)	aroE	4522139..4522957 (-)	819	WP_000451230.1	shikimate dehydrogenase	-
K8B88_RS22145 (K8B88_22060)	tsaC	4522962..4523534 (-)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
K8B88_RS22150 (K8B88_22065)	yrdD	4523539..4524081 (-)	543	WP_001312137.1	type I DNA topoisomerase	-
K8B88_RS22155 (K8B88_22070)	smg	4524110..4524583 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
K8B88_RS22160 (K8B88_22075)	dprA	4524555..4525679 (-)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
K8B88_RS22165 (K8B88_22080)	def	4525809..4526318 (+)	510	WP_000114984.1	peptide deformylase	-
K8B88_RS22170 (K8B88_22085)	fmt	4526333..4527280 (+)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
K8B88_RS22175 (K8B88_22090)	rsmB	4527332..4528621 (+)	1290	WP_000744766.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
K8B88_RS22180 (K8B88_22095)	trkA	4528643..4530019 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
K8B88_RS22185 (K8B88_22100)	mscL	4530149..4530562 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=528811 K8B88_RS22160 WP_000228517.1 4524555..4525679(-) (dprA) [Escherichia coli O16:H6 strain CU37RT-2]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=528811 K8B88_RS22160 WP_000228517.1 4524555..4525679(-) (dprA) [Escherichia coli O16:H6 strain CU37RT-2]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0528811