Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K8B88_RS20410 Genome accession   NZ_CP082774
Coordinates   4169782..4171275 (-) Length   497 a.a.
NCBI ID   WP_021524514.1    Uniprot ID   -
Organism   Escherichia coli O16:H6 strain CU37RT-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4164782..4176275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B88_RS20380 (K8B88_20305) gspK 4165576..4166553 (-) 978 WP_234247867.1 type II secretion system minor pseudopilin GspK -
  K8B88_RS20385 (K8B88_20310) gspJ 4166550..4167155 (-) 606 WP_001331694.1 type II secretion system minor pseudopilin GspJ -
  K8B88_RS20390 (K8B88_20315) gspI 4167152..4167523 (-) 372 WP_000820096.1 type II secretion system minor pseudopilin GspI -
  K8B88_RS20395 (K8B88_20320) gspH 4167520..4168083 (-) 564 WP_001115118.1 type II secretion system minor pseudopilin GspH -
  K8B88_RS20400 (K8B88_20325) gspG 4168087..4168542 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  K8B88_RS20405 (K8B88_20330) gspF 4168559..4169782 (-) 1224 WP_001173408.1 type II secretion system inner membrane protein GspF -
  K8B88_RS20410 (K8B88_20335) pilB/pilB1 4169782..4171275 (-) 1494 WP_021524514.1 type II secretion system ATPase GspE Machinery gene
  K8B88_RS20415 (K8B88_20340) gspD 4171275..4173335 (-) 2061 WP_000498844.1 type II secretion system secretin GspD -
  K8B88_RS20420 (K8B88_20345) gspC 4173365..4174324 (-) 960 WP_000135098.1 type II secretion system protein GspC -
  K8B88_RS20425 (K8B88_20350) gspS2 4174342..4174752 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  K8B88_RS20430 (K8B88_20355) pilD 4174818..4175627 (-) 810 WP_000895858.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55129.21 Da        Isoelectric Point: 5.8815

>NTDB_id=528805 K8B88_RS20410 WP_021524514.1 4169782..4171275(-) (pilB/pilB1) [Escherichia coli O16:H6 strain CU37RT-2]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLMGISDTHAVTLYHSQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=528805 K8B88_RS20410 WP_021524514.1 4169782..4171275(-) (pilB/pilB1) [Escherichia coli O16:H6 strain CU37RT-2]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ATGCGCCCTTTACACTGCGGCAAATCGACGAGGCTGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGTTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGAG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGTCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATTCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Legionella pneumophila strain ERS1305867

43.155

86.72

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36