Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K8B88_RS20430 Genome accession   NZ_CP082774
Coordinates   4174818..4175627 (-) Length   269 a.a.
NCBI ID   WP_000895858.1    Uniprot ID   -
Organism   Escherichia coli O16:H6 strain CU37RT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4169818..4180627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B88_RS20415 (K8B88_20340) gspD 4171275..4173335 (-) 2061 WP_000498844.1 type II secretion system secretin GspD -
  K8B88_RS20420 (K8B88_20345) gspC 4173365..4174324 (-) 960 WP_000135098.1 type II secretion system protein GspC -
  K8B88_RS20425 (K8B88_20350) gspS2 4174342..4174752 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  K8B88_RS20430 (K8B88_20355) pilD 4174818..4175627 (-) 810 WP_000895858.1 prepilin peptidase PppA Machinery gene
  K8B88_RS20435 (K8B88_20360) sslE 4175691..4180247 (-) 4557 WP_234247873.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29419.00 Da        Isoelectric Point: 8.3801

>NTDB_id=528806 K8B88_RS20430 WP_000895858.1 4174818..4175627(-) (pilD) [Escherichia coli O16:H6 strain CU37RT-2]
MLFDVFQQYPAAMPILATAGGLIIGSFLNVVIWRYPIMLRQQMAEFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGMATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=528806 K8B88_RS20430 WP_000895858.1 4174818..4175627(-) (pilD) [Escherichia coli O16:H6 strain CU37RT-2]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCATACTGGCAACCGCAGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAACGCCGAGTACGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCATGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTTGAGTT
ATTGACAGCACTTGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CGTGGCTGATTGCCGCGAGCATCATCGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGTTAACGCTACAAGACGCAGTCACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCCG
CTTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGTCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATGGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

41.026

100

0.416
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372