Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K7X33_RS03780 Genome accession   NZ_CP082355
Coordinates   799171..800664 (+) Length   497 a.a.
NCBI ID   WP_000249332.1    Uniprot ID   -
Organism   Escherichia coli strain VCSW5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794171..805664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X33_RS03760 (K7X33_03760) pilD 794819..795628 (+) 810 WP_000464641.1 prepilin peptidase PppA Machinery gene
  K7X33_RS03765 (K7X33_03765) gspS2 795694..796104 (+) 411 WP_001305972.1 type II secretion system pilot lipoprotein GspS-beta -
  K7X33_RS03770 (K7X33_03770) gspC 796122..797081 (+) 960 WP_001308941.1 type II secretion system protein GspC -
  K7X33_RS03775 (K7X33_03775) gspD 797111..799171 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  K7X33_RS03780 (K7X33_03780) pilB/pilB1 799171..800664 (+) 1494 WP_000249332.1 type II secretion system ATPase GspE Machinery gene
  K7X33_RS03785 (K7X33_03785) gspF 800664..801887 (+) 1224 WP_001173428.1 type II secretion system inner membrane protein GspF -
  K7X33_RS03790 (K7X33_03790) gspG 801904..802359 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  K7X33_RS03795 (K7X33_03795) gspH 802363..802926 (+) 564 WP_001115145.1 type II secretion system minor pseudopilin GspH -
  K7X33_RS03800 (K7X33_03800) gspI 802923..803294 (+) 372 WP_000820102.1 type II secretion system minor pseudopilin GspI -
  K7X33_RS03805 (K7X33_03805) gspJ 803291..803896 (+) 606 WP_001308939.1 type II secretion system minor pseudopilin GspJ -
  K7X33_RS03810 (K7X33_03810) gspK 803893..804870 (+) 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55244.38 Da        Isoelectric Point: 6.0089

>NTDB_id=528337 K7X33_RS03780 WP_000249332.1 799171..800664(+) (pilB/pilB1) [Escherichia coli strain VCSW5]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQSCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=528337 K7X33_RS03780 WP_000249332.1 799171..800664(+) (pilB/pilB1) [Escherichia coli strain VCSW5]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTTTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTAGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGTG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTCTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGCCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCGCGTACCGTCACTCTGTACCATCCGCAGAGCTGTCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACTGCCATCCATGAGCTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACTATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.707

79.678

0.364
  pilF Thermus thermophilus HB27

44.75

80.483

0.36