Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   K7X33_RS01725 Genome accession   NZ_CP082355
Coordinates   392986..394224 (+) Length   412 a.a.
NCBI ID   WP_000815960.1    Uniprot ID   -
Organism   Escherichia coli strain VCSW5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 387986..399224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X33_RS01700 (K7X33_01700) mrcA 388266..390818 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  K7X33_RS01705 (K7X33_01705) hofM 390938..391717 (+) 780 WP_001309005.1 DNA utilization protein HofM -
  K7X33_RS01710 (K7X33_01710) hofN 391717..392256 (+) 540 WP_001069322.1 DNA utilization protein HofN -
  K7X33_RS01715 (K7X33_01715) hofO 392240..392680 (+) 441 WP_000367367.1 DNA utilization protein HofO -
  K7X33_RS01720 (K7X33_01720) hofP 392670..393074 (+) 405 WP_001309004.1 DNA utilization protein HofP -
  K7X33_RS01725 (K7X33_01725) comE 392986..394224 (+) 1239 WP_000815960.1 DNA uptake porin HofQ Machinery gene
  K7X33_RS01730 (K7X33_01730) aroK 394625..395146 (+) 522 WP_000818618.1 shikimate kinase AroK -
  K7X33_RS01735 (K7X33_01735) aroB 395203..396291 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  K7X33_RS01740 (K7X33_01740) damX 396383..397669 (+) 1287 WP_000343221.1 cell division protein DamX -
  K7X33_RS01745 (K7X33_01745) dam 397776..398612 (+) 837 WP_000742139.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44675.18 Da        Isoelectric Point: 6.3191

>NTDB_id=528327 K7X33_RS01725 WP_000815960.1 392986..394224(+) (comE) [Escherichia coli strain VCSW5]
MKQWIAALLLMLIPGAQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLKYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=528327 K7X33_RS01725 WP_000815960.1 392986..394224(+) (comE) [Escherichia coli strain VCSW5]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGCACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCATGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGAAATACGCCGACGCGGGAGAACTGGCGA
AAGCCGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTACAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362