Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I6I51_RS10210 Genome accession   NZ_CP068187
Coordinates   1953222..1953944 (-) Length   240 a.a.
NCBI ID   WP_004691497.1    Uniprot ID   A0A380U8X3
Organism   Acinetobacter johnsonii strain FDAARGOS_1094     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948222..1958944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I51_RS10190 (I6I51_10195) aroB 1948910..1949995 (-) 1086 WP_125280900.1 3-dehydroquinate synthase -
  I6I51_RS10195 (I6I51_10200) aroK 1950026..1950559 (-) 534 WP_201701765.1 shikimate kinase AroK -
  I6I51_RS10200 (I6I51_10205) pilQ 1950597..1952675 (-) 2079 WP_201701767.1 type IV pilus secretin PilQ family protein Machinery gene
  I6I51_RS10205 (I6I51_10210) pilP 1952695..1953222 (-) 528 WP_004691496.1 pilus assembly protein PilP Machinery gene
  I6I51_RS10210 (I6I51_10215) pilO 1953222..1953944 (-) 723 WP_004691497.1 type 4a pilus biogenesis protein PilO Machinery gene
  I6I51_RS10215 (I6I51_10220) pilN 1953941..1954597 (-) 657 WP_168158668.1 PilN domain-containing protein Machinery gene
  I6I51_RS10220 (I6I51_10225) pilM 1954597..1955655 (-) 1059 WP_201701769.1 pilus assembly protein PilM Machinery gene
  I6I51_RS10225 (I6I51_10230) ponA 1955821..1958349 (+) 2529 WP_201701771.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27004.43 Da        Isoelectric Point: 4.9937

>NTDB_id=527900 I6I51_RS10210 WP_004691497.1 1953222..1953944(-) (pilO) [Acinetobacter johnsonii strain FDAARGOS_1094]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide


Download         Length: 723 bp        

>NTDB_id=527900 I6I51_RS10210 WP_004691497.1 1953222..1953944(-) (pilO) [Acinetobacter johnsonii strain FDAARGOS_1094]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCGCAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCGCAGTTGCAAGAAAT
GGAAGCTAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAACATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCTATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380U8X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

72.385

99.583

0.721
  comO Acinetobacter baylyi ADP1

67.755

100

0.692