NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	I6J12_RS00045	Genome accession	NZ_CP068060
Coordinates	4221..4658 (+)	Length	145 a.a.
NCBI ID	WP_003035329.1	Uniprot ID	F9P803
Organism	Streptococcus anginosus strain FDAARGOS_1155
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	57..6003	4221..4658	within	0

Gene organization within MGE regions

Location: 57..6003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J12_RS00010 (I6J12_00010)	-	845..1387 (+)	543	WP_003038192.1	DUF1366 domain-containing protein	-
I6J12_RS00015 (I6J12_00015)	-	1405..1806 (+)	402	WP_003038210.1	hypothetical protein	-
I6J12_RS00020 (I6J12_00020)	-	1808..2140 (+)	333	WP_003038184.1	phage holin	-
I6J12_RS00025 (I6J12_00025)	-	2143..2892 (+)	750	WP_003038204.1	PlySs2 family phage lysin	-
I6J12_RS00030 (I6J12_00030)	comYC	3315..3584 (+)	270	WP_003035361.1	competence type IV pilus major pilin ComGC	Machinery gene
I6J12_RS00035 (I6J12_00035)	comYD	3544..3972 (+)	429	WP_020999411.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6J12_RS00040 (I6J12_00040)	comGE/cglE	3944..4237 (+)	294	WP_003035318.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6J12_RS00045 (I6J12_00045)	comGF/cglF	4221..4658 (+)	438	WP_003035329.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6J12_RS00050 (I6J12_00050)	comGG	4639..4965 (+)	327	WP_020999447.1	competence type IV pilus minor pilin ComGG	-
I6J12_RS00055 (I6J12_00055)	comYH	5050..6003 (+)	954	WP_003035359.1	class I SAM-dependent methyltransferase	Machinery gene

Sequence

Protein

Download Length: 145 a.a. Molecular weight: 16865.57 Da Isoelectric Point: 10.6422

                                >NTDB_id=526767 I6J12_RS00045 WP_003035329.1 4221..4658(+) (comGF/cglF) [Streptococcus anginosus strain FDAARGOS_1155]

MFKANKVKAFTLLEALVALFVISGSVLLFQSMTRLLASEVRARQHSEQREWLLFAHQLEAELIRSSFEKVENNRLYMKQD

GKTIAFGKSSGQDFRKTNANGKGYQPMIYNVKKAEISQKNELIHIRMTFKRGLEREFVYRVETKS

Nucleotide

Download Length: 438 bp

                                >NTDB_id=526767 I6J12_RS00045 WP_003035329.1 4221..4658(+) (comGF/cglF) [Streptococcus anginosus strain FDAARGOS_1155]

GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACACTTTTAGAGGCTCTGGTTGCCCTCTTTGTAATCAGCGGGAGTGTGCT

ATTATTTCAATCTATGACGCGTCTTCTGGCTTCGGAGGTCCGAGCTCGACAACACAGTGAACAACGAGAATGGCTTTTGT

TTGCCCATCAATTAGAAGCGGAATTGATTCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAGGAC

GGTAAGACGATTGCATTTGGAAAGTCAAGTGGACAAGATTTCAGAAAAACCAATGCAAATGGGAAAGGCTATCAGCCTAT

GATTTATAATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATATCAGAATGACATTTAAGCGAGGATTGG

AAAGGGAGTTCGTCTATCGTGTGGAAACAAAGAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(39-134)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	F9P803

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae R6	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae D39	66.429	96.552	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.972	97.241	0.428
comYF	Streptococcus mutans UA140	43.972	97.241	0.428
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4

Detailed information NTG0526767