Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JJQ97_RS02045 Genome accession   NZ_CP068034
Coordinates   446830..447894 (+) Length   354 a.a.
NCBI ID   WP_003414236.1    Uniprot ID   A0A0P9RSF6
Organism   Pseudomonas syringae strain BIM B-268     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441830..452894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJQ97_RS02035 (JJQ97_01680) - 442694..443962 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -
  JJQ97_RS02040 (JJQ97_01685) - 444179..446623 (-) 2445 WP_003414235.1 penicillin-binding protein 1A -
  JJQ97_RS02045 (JJQ97_01690) pilM 446830..447894 (+) 1065 WP_003414236.1 type IV pilus assembly protein PilM Machinery gene
  JJQ97_RS02050 (JJQ97_01695) - 447894..448469 (+) 576 WP_201419117.1 PilN domain-containing protein -
  JJQ97_RS02055 (JJQ97_01700) pilO 448466..449089 (+) 624 WP_017278462.1 type 4a pilus biogenesis protein PilO -
  JJQ97_RS02060 (JJQ97_01705) pilP 449086..449613 (+) 528 WP_201419118.1 type 4a pilus biogenesis lipoprotein PilP -
  JJQ97_RS02065 (JJQ97_01710) pilQ 449661..451766 (+) 2106 WP_201419119.1 type IV pilus secretin PilQ Machinery gene
  JJQ97_RS02070 (JJQ97_01715) aroK 451771..452289 (+) 519 WP_024684306.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38120.65 Da        Isoelectric Point: 4.7049

>NTDB_id=526440 JJQ97_RS02045 WP_003414236.1 446830..447894(+) (pilM) [Pseudomonas syringae strain BIM B-268]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=526440 JJQ97_RS02045 WP_003414236.1 446830..447894(+) (pilM) [Pseudomonas syringae strain BIM B-268]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCGTTCGGTTTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAGGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGGCGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGACGCGCCGGCGCTGATGATCGCGTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9RSF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466