NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	K6135_RS17405	Genome accession	NZ_CP082094
Coordinates	3621406..3622530 (-)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	A0A0H3EME5
Organism	Escherichia coli strain MS15236
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3616406..3627530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6135_RS17375 (K6135_17220)	yrdA	3618206..3618760 (+)	555	WP_001541769.1	gamma carbonic anhydrase family protein	-
K6135_RS17380 (K6135_17225)	yrdB	3618736..3618993 (-)	258	WP_001070580.1	DUF1488 domain-containing protein	-
K6135_RS17385 (K6135_17230)	aroE	3618990..3619808 (-)	819	WP_000451224.1	shikimate dehydrogenase	-
K6135_RS17390 (K6135_17235)	tsaC	3619813..3620385 (-)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
K6135_RS17395 (K6135_17240)	yrdD	3620390..3620932 (-)	543	WP_001129723.1	type I DNA topoisomerase	-
K6135_RS17400 (K6135_17245)	smg	3620961..3621434 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
K6135_RS17405 (K6135_17250)	dprA	3621406..3622530 (-)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
K6135_RS17410 (K6135_17255)	def	3622660..3623169 (+)	510	WP_000114984.1	peptide deformylase	-
K6135_RS17415 (K6135_17260)	fmt	3623184..3624131 (+)	948	WP_000004426.1	methionyl-tRNA formyltransferase	-
K6135_RS17420 (K6135_17265)	rsmB	3624183..3625472 (+)	1290	WP_000744767.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
K6135_RS17425 (K6135_17270)	trkA	3625494..3626870 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
K6135_RS17430 (K6135_17275)	mscL	3627000..3627413 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=525975 K6135_RS17405 WP_000228517.1 3621406..3622530(-) (dprA) [Escherichia coli strain MS15236]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=525975 K6135_RS17405 WP_000228517.1 3621406..3622530(-) (dprA) [Escherichia coli strain MS15236]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0525975