Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JHW38_RS11250 Genome accession   NZ_CP067395
Coordinates   2710224..2711951 (+) Length   575 a.a.
NCBI ID   WP_207525988.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705224..2716951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS11225 (JHW38_11225) - 2705469..2706107 (+) 639 WP_207525983.1 class I SAM-dependent methyltransferase -
  JHW38_RS11230 (JHW38_11230) - 2706104..2707168 (+) 1065 WP_207525984.1 NAD(P)-binding domain-containing protein -
  JHW38_RS11235 (JHW38_11235) - 2707151..2707996 (-) 846 WP_207525985.1 GNAT family N-acetyltransferase -
  JHW38_RS11240 (JHW38_11240) - 2707989..2709137 (-) 1149 WP_207525986.1 glycosyltransferase -
  JHW38_RS11245 (JHW38_11245) - 2709226..2710089 (-) 864 WP_207525987.1 glycosyltransferase family 2 protein -
  JHW38_RS11250 (JHW38_11250) pilB 2710224..2711951 (+) 1728 WP_207525988.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JHW38_RS11255 (JHW38_11255) pilC 2711955..2713223 (+) 1269 WP_207525989.1 type II secretion system F family protein Machinery gene
  JHW38_RS11260 (JHW38_11260) - 2713243..2714130 (+) 888 WP_207525990.1 A24 family peptidase -
  JHW38_RS11265 (JHW38_11265) coaE 2714130..2714744 (+) 615 WP_207525991.1 dephospho-CoA kinase -
  JHW38_RS11270 (JHW38_11270) - 2714951..2715922 (-) 972 WP_278249820.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62486.74 Da        Isoelectric Point: 6.0993

>NTDB_id=525851 JHW38_RS11250 WP_207525988.1 2710224..2711951(+) (pilB) [Lysobacter enzymogenes strain CX03]
MSTATANLVGITGIARRLVMDGALDEVKAREAMAAASAERKPLASYLAENRLATSAQLAAANSIEFGVPLFDPTAMDPNQ
SASRLISDELVRKHNALPLFKRGNKLFIGLADPTNSRALDEIKFQTNLVIEAILVDEALIKSTLDRWTETNDTLGDAVGD
GEGLDTLEVGKDDEGLGAGGDTGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQRDLF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDDQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEIAAGIALFEPVGCPDCTEGYKGRTGIYQVMPMTEEIQAIVLEGGNAMQIAAAATRSGVRDLRRSALVKVL
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=525851 JHW38_RS11250 WP_207525988.1 2710224..2711951(+) (pilB) [Lysobacter enzymogenes strain CX03]
ATGTCCACCGCTACTGCCAACCTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGACGGCGCCCTGGACGAGGT
CAAGGCCCGCGAAGCGATGGCCGCGGCCAGCGCCGAGCGCAAGCCGCTGGCCTCATACCTGGCTGAGAACCGCCTGGCGA
CCTCGGCCCAGCTGGCCGCCGCCAACTCCATCGAATTCGGCGTGCCGCTGTTCGATCCGACCGCGATGGATCCGAACCAG
TCGGCCTCGCGCCTGATCAGCGACGAACTGGTGCGCAAGCACAACGCGCTGCCGCTGTTCAAGCGCGGCAACAAGCTGTT
CATCGGCCTGGCCGATCCGACCAACAGCCGCGCGCTCGACGAGATCAAGTTCCAGACCAATCTGGTCATCGAAGCGATCC
TGGTCGACGAGGCCCTGATCAAGTCGACCCTCGACCGCTGGACCGAAACCAACGACACCCTCGGCGACGCGGTCGGCGAC
GGCGAAGGCCTGGACACCCTGGAGGTCGGCAAGGACGACGAGGGCCTGGGCGCCGGCGGCGACACCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAACCGAATACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTCCCGAACAAG
CTGCATTCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCGGCCGCGCGCCTGGGCATCGAAAAGCTCGGCTACGAGGACGACCAGCGCGACCTGTTC
GTCAACGCGGTGGTCAAGCCTTACGGCATGGTCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGAACATCCTCAACGACGACCAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCCGAAATCGCCATCAAGGCCGCGCAGACCGGCCATATGGTGCTGTC
GACCCTGCACACCAACGACGCCCCGCAGACCATCGCGCGCCTCATGAACATGGGCGTGGCGCCGTACAACATCACCTCCT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCCCGGCGCCTGCACGACTGCAAGCGCGAGGTCCACCTGCCGGAACACGCG
CTGCTGGCCGAAGGCTTCACCCACGAAGAAATCGCCGCCGGCATCGCCCTCTTTGAACCGGTCGGCTGCCCCGACTGCAC
CGAGGGCTACAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGACCGAAGAAATCCAGGCGATCGTGCTCGAAGGCG
GCAACGCGATGCAGATCGCCGCCGCCGCCACCCGGTCCGGCGTGCGCGACCTGCGCCGCTCGGCCCTGGTCAAGGTGCTC
AACGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.641

99.304

0.543
  pilB Acinetobacter baumannii D1279779

54.482

98.957

0.539
  pilB Legionella pneumophila strain ERS1305867

50.175

99.478

0.499
  pilB Vibrio cholerae strain A1552

50.775

89.739

0.456
  pilF Neisseria gonorrhoeae MS11

45.709

99.304

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

49.143

91.304

0.449
  pilB Vibrio campbellii strain DS40M4

51.016

85.565

0.437