NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	K6125_RS19590	Genome accession	NZ_CP082077
Coordinates	4021975..4023099 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	A0A0H3EME5
Organism	Escherichia coli strain MS20657
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 4016975..4028099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6125_RS19565 (K6125_19450)	mscL	4017092..4017505 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
K6125_RS19570 (K6125_19455)	trkA	4017635..4019011 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
K6125_RS19575 (K6125_19460)	rsmB	4019033..4020322 (-)	1290	WP_000744767.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
K6125_RS19580 (K6125_19465)	fmt	4020374..4021321 (-)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
K6125_RS19585 (K6125_19470)	def	4021336..4021845 (-)	510	WP_000114984.1	peptide deformylase	-
K6125_RS19590 (K6125_19475)	dprA	4021975..4023099 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
K6125_RS19595 (K6125_19480)	smg	4023071..4023544 (+)	474	WP_001553514.1	DUF494 family protein Smg	-
K6125_RS19600 (K6125_19485)	yrdD	4023573..4024115 (+)	543	WP_001553513.1	type I DNA topoisomerase	-
K6125_RS19605 (K6125_19490)	tsaC	4024120..4024692 (+)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
K6125_RS19610 (K6125_19495)	aroE	4024697..4025515 (+)	819	WP_001553512.1	shikimate dehydrogenase	-
K6125_RS19615 (K6125_19500)	yrdB	4025512..4025769 (+)	258	WP_001070581.1	DUF1488 domain-containing protein	-
K6125_RS19620 (K6125_19505)	yrdA	4025745..4026299 (-)	555	WP_001286206.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=525488 K6125_RS19590 WP_000228517.1 4021975..4023099(+) (dprA) [Escherichia coli strain MS20657]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=525488 K6125_RS19590 WP_000228517.1 4021975..4023099(+) (dprA) [Escherichia coli strain MS20657]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAGGGGGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0525488