NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	K6125_RS19085	Genome accession	NZ_CP082077
Coordinates	3940149..3941387 (+)	Length	412 a.a.
NCBI ID	WP_071600678.1	Uniprot ID	-
Organism	Escherichia coli strain MS20657
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3935149..3946387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6125_RS19060 (K6125_18945)	mrcA	3935429..3937981 (-)	2553	WP_001298208.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-
K6125_RS19065 (K6125_18950)	hofM	3938101..3938880 (+)	780	WP_001296474.1	DNA utilization protein HofM	-
K6125_RS19070 (K6125_18955)	hofN	3938880..3939419 (+)	540	WP_001069330.1	DNA utilization protein HofN	-
K6125_RS19075 (K6125_18960)	hofO	3939403..3939843 (+)	441	WP_001055745.1	DNA utilization protein HofO	-
K6125_RS19080 (K6125_18965)	hofP	3939833..3940237 (+)	405	WP_001264137.1	DNA utilization protein HofP	-
K6125_RS19085 (K6125_18970)	comE	3940149..3941387 (+)	1239	WP_071600678.1	DNA uptake porin HofQ	Machinery gene
K6125_RS19090 (K6125_18975)	aroK	3941788..3942309 (+)	522	WP_000818618.1	shikimate kinase AroK	-
K6125_RS19095 (K6125_18980)	aroB	3942366..3943454 (+)	1089	WP_001356903.1	3-dehydroquinate synthase	-
K6125_RS19100 (K6125_18985)	damX	3943546..3944832 (+)	1287	WP_000343172.1	cell division protein DamX	-
K6125_RS19105 (K6125_18990)	dam	3944939..3945775 (+)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44855.30 Da Isoelectric Point: 5.7077

                                >NTDB_id=525484 K6125_RS19085 WP_071600678.1 3940149..3941387(+) (comE) [Escherichia coli strain MS20657]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSDQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=525484 K6125_RS19085 WP_071600678.1 3940149..3941387(+) (comE) [Escherichia coli strain MS20657]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGATCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367
comE	Glaesserella parasuis strain SC1401	35.545	100	0.364

Detailed information NTG0525484