NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	K6O12_RS18005	Genome accession	NZ_CP082075
Coordinates	3715357..3716595 (-)	Length	412 a.a.
NCBI ID	WP_000816009.1	Uniprot ID	-
Organism	Escherichia coli strain MS20658
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3710357..3721595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6O12_RS17985 (K6O12_17890)	dam	3710969..3711805 (-)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-
K6O12_RS17990 (K6O12_17895)	damX	3711912..3713198 (-)	1287	WP_000343173.1	cell division protein DamX	-
K6O12_RS17995 (K6O12_17900)	aroB	3713290..3714378 (-)	1089	WP_000439850.1	3-dehydroquinate synthase	-
K6O12_RS18000 (K6O12_17905)	aroK	3714435..3714956 (-)	522	WP_000818618.1	shikimate kinase AroK	-
K6O12_RS18005 (K6O12_17910)	comE	3715357..3716595 (-)	1239	WP_000816009.1	DNA uptake porin HofQ	Machinery gene
K6O12_RS18010 (K6O12_17915)	hofP	3716507..3716911 (-)	405	WP_302359694.1	DNA utilization protein HofP	-
K6O12_RS18015 (K6O12_17920)	hofO	3716901..3717341 (-)	441	WP_001055744.1	DNA utilization protein HofO	-
K6O12_RS18020 (K6O12_17925)	hofN	3717325..3717864 (-)	540	Protein_3533	DNA utilization protein HofN	-
K6O12_RS18025 (K6O12_17930)	hofM	3717864..3718643 (-)	780	WP_001308095.1	DNA utilization protein HofM	-
K6O12_RS18030 (K6O12_17935)	mrcA	3718763..3721315 (+)	2553	WP_001298208.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44811.29 Da Isoelectric Point: 5.9488

                                >NTDB_id=525391 K6O12_RS18005 WP_000816009.1 3715357..3716595(-) (comE) [Escherichia coli strain MS20658]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNVARQEVEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=525391 K6O12_RS18005 WP_000816009.1 3715357..3716595(-) (comE) [Escherichia coli strain MS20658]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATGTCGCCCGCCAGGAAGTGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae Rd KW20	37.385	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae 86-028NP	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
pilQ	Pseudomonas aeruginosa PAK	35.092	100	0.371
comE	Glaesserella parasuis strain SC1401	35.782	100	0.367

Detailed information NTG0525391