Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH287_RS13825 Genome accession   NZ_CP067001
Coordinates   3135508..3137241 (-) Length   577 a.a.
NCBI ID   WP_274497103.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 40-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3130508..3142241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH287_RS13815 (JH287_13810) - 3131877..3133487 (+) 1611 WP_011038210.1 HAMP domain-containing sensor histidine kinase -
  JH287_RS13820 (JH287_13815) pilR 3133696..3135090 (+) 1395 WP_165349438.1 sigma-54 dependent transcriptional regulator Regulator
  JH287_RS13825 (JH287_13820) pilB 3135508..3137241 (-) 1734 WP_274497103.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH287_RS13830 (JH287_13825) pilA/pilAI 3137362..3137769 (-) 408 WP_129588891.1 pilin Machinery gene
  JH287_RS13835 (JH287_13830) pilA/pilAI 3137844..3138254 (-) 411 WP_274497102.1 pilin Machinery gene
  JH287_RS13840 (JH287_13835) pilC 3138614..3139870 (+) 1257 WP_129589289.1 type II secretion system F family protein Machinery gene
  JH287_RS13845 (JH287_13840) - 3139877..3140740 (+) 864 WP_129588889.1 A24 family peptidase -
  JH287_RS13850 (JH287_13845) coaE 3140754..3141374 (+) 621 WP_129588888.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62795.97 Da        Isoelectric Point: 5.6058

>NTDB_id=524121 JH287_RS13825 WP_274497103.1 3135508..3137241(-) (pilB) [Xanthomonas campestris pv. campestris strain 40-2]
MSAVTSANLVGITGIARRLVQDGALEEATARAAMDQATAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGASMG
DGDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTQEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=524121 JH287_RS13825 WP_274497103.1 3135508..3137241(-) (pilB) [Xanthomonas campestris pv. campestris strain 40-2]
ATGAGCGCAGTCACTTCCGCTAACTTGGTCGGTATAACCGGCATAGCCAGACGGCTAGTTCAGGACGGTGCGTTGGAAGA
GGCGACAGCGCGTGCTGCAATGGACCAGGCTACGGCAGCAAAGATTCCCCTGCCGCAATGGTTCTCTGAGAAGAAGTTGG
TCACCGCATCCCAGCTCGCTGCCGCCAACGCAGTCGAGTTCGGCATGCCGCTGATGGATGTGTCGGTGTTCGATGCCAGT
CAGAATGCAGTCAAGCTGGTGAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTCGTGGGGGTGAGCAATCCAACCCAGACTCGAGCGCTGGACGATATTAAGTTCCATACGAATCTGGTTGTCGAACCGA
TCCTCGTCGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAAGCCAGCAATGCGTCGCTTGGCGCGTCGATGGGC
GACGGCGATGATGAGATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAGATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCTTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGTAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCAGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.786

100

0.56
  pilB Acinetobacter baylyi ADP1

54.922

100

0.551
  pilB Legionella pneumophila strain ERS1305867

52.197

98.614

0.515
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio cholerae strain A1552

50

94.974

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

46.858

96.534

0.452
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451