Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH281_RS03005 Genome accession   NZ_CP066919
Coordinates   700576..702309 (-) Length   577 a.a.
NCBI ID   WP_227971327.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain CFBP6943     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 694127..706399 700576..702309 within 0


Gene organization within MGE regions


Location: 694127..706399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH281_RS02980 (JH281_02965) - 694127..694537 (-) 411 WP_029628876.1 DNA-binding protein -
  JH281_RS02985 (JH281_02970) sucD 694641..695516 (-) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH281_RS02990 (JH281_02975) sucC 695541..696710 (-) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  JH281_RS02995 (JH281_02980) - 696944..698554 (+) 1611 WP_040940804.1 HAMP domain-containing sensor histidine kinase -
  JH281_RS03000 (JH281_02985) pilR 698764..700158 (+) 1395 WP_042594919.1 sigma-54 dependent transcriptional regulator Regulator
  JH281_RS03005 (JH281_02990) pilB 700576..702309 (-) 1734 WP_227971327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH281_RS03010 (JH281_02995) - 702349..702759 (-) 411 WP_274316877.1 pilin -
  JH281_RS03015 (JH281_03000) - 702848..703264 (-) 417 WP_227971328.1 pilin -
  JH281_RS03020 (JH281_03005) pilC 703690..704892 (+) 1203 WP_227971345.1 type II secretion system F family protein Machinery gene
  JH281_RS03025 (JH281_03010) - 704899..705762 (+) 864 WP_040940797.1 A24 family peptidase -
  JH281_RS03030 (JH281_03015) coaE 705776..706399 (+) 624 WP_274316878.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62805.97 Da        Isoelectric Point: 5.7640

>NTDB_id=523164 JH281_RS03005 WP_227971327.1 700576..702309(-) (pilB) [Xanthomonas campestris pv. campestris strain CFBP6943]
MNVANSPNLVGITGIARRLVQDGALEEAAARTAMDQATAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTQEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523164 JH281_RS03005 WP_227971327.1 700576..702309(-) (pilB) [Xanthomonas campestris pv. campestris strain CFBP6943]
ATGAACGTGGCTAATTCTCCCAATCTAGTTGGAATTACTGGAATTGCTCGTCGTTTAGTCCAAGACGGAGCGTTGGAAGA
GGCTGCCGCTCGCACCGCAATGGACCAGGCTACGGCAGCCAAAATTCCCTTGCCGCAATGGTTCTCTGAGAAGAAGTTGG
TCACCGCATCCCAGCTCGCTGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGATGGATGTGTCGGTGTTCGATGCCAGT
CAGAATGCAGTCAAGCTGGTGAGTGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGA
TCCTCGTCGACGAGGATCAGATCCGGCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGG
GACGACGACGAGGGGATGGGAGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCACTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGTTGTTGAAGAACGTAGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAACTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGTTACGAGGCAGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCACTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCTTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGTAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCAGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

55.106

98.44

0.542
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.126

99.133

0.487
  pilF Neisseria gonorrhoeae MS11

48.592

98.44

0.478
  pilB Vibrio campbellii strain DS40M4

46.221

98.614

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.869

96.88

0.454