Detailed information NTG0052310

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MRBBS_RS03255
• Genome accession: NC_018268
• Coordinates: 723268..724332 (+)
• Length: 354 a.a.
• NCBI ID: WP_014869993.1
• Uniprot ID: M1FA40
• Organism: Marinobacter sp. BSs20148
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 718268..729332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MRBBS_RS03240 (MRBBS_0689)	rpmE	718669..718893 (+)	225	WP_014869990.1	50S ribosomal protein L31	-
MRBBS_RS03245 (MRBBS_0690)	-	719143..720408 (+)	1266	WP_014869991.1	malic enzyme-like NAD(P)-binding protein	-
MRBBS_RS03250 (MRBBS_0691)	-	720537..723074 (-)	2538	WP_014869992.1	penicillin-binding protein 1A	-
MRBBS_RS03255 (MRBBS_0692)	pilM	723268..724332 (+)	1065	WP_014869993.1	pilus assembly protein PilM	Machinery gene
MRBBS_RS03260 (MRBBS_0693)	-	724332..724898 (+)	567	WP_014869994.1	PilN domain-containing protein	-
MRBBS_RS03265 (MRBBS_0694)	-	724895..725506 (+)	612	WP_014869995.1	type 4a pilus biogenesis protein PilO	-
MRBBS_RS03270 (MRBBS_0695)	-	725509..726081 (+)	573	WP_014869996.1	pilus assembly protein PilP	-
MRBBS_RS03275 (MRBBS_0696)	pilQ	726081..728198 (+)	2118	WP_014869997.1	type IV pilus secretin PilQ	Machinery gene
MRBBS_RS03280 (MRBBS_0697)	aroK	728338..728904 (+)	567	WP_014869998.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37888.86 Da
• Isoelectric Point: 4.2419

>NTDB_id=52310 MRBBS_RS03255 WP_014869993.1 723268..724332(+) (pilM) [Marinobacter sp. BSs20148]
MFGSFGKKSSGVLGLDISSSSVKLLELSKQGDRFRVESYAVEPLPANAVVEKNITDVEAVGDAIKRVVSKSRSGVKQVAV
AVSGSAVITKVIQMDGGINEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDKVDVLIAASRKENVDVRQDALNI
AGLTTKVVDVEAYALERAYQLIESQLNSHGDDLVVAIMDVGATMATLSVIAEGKTVYTREQVFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYEAEVLNPFREAVVQQVARAMQFFFGASQYNSVDYVVLAGGTSAIPGLVDMVEEKTGTPTLVANP
FADMAVSSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=52310 MRBBS_RS03255 WP_014869993.1 723268..724332(+) (pilM) [Marinobacter sp. BSs20148]
GTGTTTGGATCTTTCGGAAAGAAATCCAGTGGTGTGCTGGGCTTGGACATAAGCTCAAGCTCGGTCAAGCTTTTGGAATT
GTCAAAACAGGGCGACCGCTTTCGGGTTGAAAGCTATGCCGTCGAACCTTTACCTGCAAATGCTGTGGTGGAAAAAAACA
TCACGGATGTGGAAGCCGTAGGTGATGCGATCAAGCGCGTGGTGTCCAAATCCCGCTCTGGCGTCAAGCAAGTAGCCGTT
GCCGTATCCGGTTCCGCGGTGATCACCAAGGTGATCCAGATGGATGGCGGTATCAATGAATTCGAAATGGAAGACCAGAT
CGCCCTGGAGGCAGATCAATACATTCCTTATCCGCTGGATGAAGTCGCGATTGATTTCGAGGTACAAGGTCCCTCTGAAT
CCAATCCGGATAAGGTTGATGTACTGATTGCTGCCAGCCGCAAAGAGAACGTTGATGTGCGCCAAGACGCACTGAACATT
GCCGGACTGACCACCAAAGTGGTTGACGTAGAGGCTTATGCCCTCGAGCGTGCCTATCAACTGATTGAATCGCAATTGAA
CTCTCACGGCGACGATCTGGTCGTGGCGATCATGGATGTGGGCGCCACCATGGCCACGTTGAGCGTGATTGCCGAAGGCA
AAACTGTGTATACCCGCGAACAGGTGTTTGGTGGCAAGCAGTTAACTGAAGAAATCCAGCGCCGCTATGGTTTGTCGCTG
GAGGAAGCCGGCCTGGCCAAAAAGCAAGGTGGCTTGCCCGATGATTATGAAGCCGAAGTGCTGAACCCGTTCCGCGAGGC
TGTGGTGCAGCAGGTCGCTCGGGCGATGCAGTTCTTTTTCGGTGCCAGCCAGTACAACTCGGTGGATTATGTGGTTTTGG
CCGGCGGCACCTCGGCAATACCCGGGCTTGTTGACATGGTAGAGGAAAAAACGGGTACGCCCACGCTGGTAGCCAATCCG
TTTGCGGACATGGCTGTTAGCTCGCGGGTCAATGCGTCGGCGTTGAGTAACGATGCTCCTTCGTTGATGATCGCGTGTGG
TCTGGCAATGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: M1FA40

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	52.26	100	0.523
comM	Acinetobacter nosocomialis M2	52.26	100	0.523
comM	Acinetobacter baylyi ADP1	51.412	100	0.514
pilM	Legionella pneumophila strain ERS1305867	45.48	100	0.455