Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MRBBS_RS03275 Genome accession   NC_018268
Coordinates   726081..728198 (+) Length   705 a.a.
NCBI ID   WP_014869997.1    Uniprot ID   M1FCB7
Organism   Marinobacter sp. BSs20148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721081..733198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRBBS_RS03255 (MRBBS_0692) pilM 723268..724332 (+) 1065 WP_014869993.1 pilus assembly protein PilM Machinery gene
  MRBBS_RS03260 (MRBBS_0693) - 724332..724898 (+) 567 WP_014869994.1 PilN domain-containing protein -
  MRBBS_RS03265 (MRBBS_0694) - 724895..725506 (+) 612 WP_014869995.1 type 4a pilus biogenesis protein PilO -
  MRBBS_RS03270 (MRBBS_0695) - 725509..726081 (+) 573 WP_014869996.1 pilus assembly protein PilP -
  MRBBS_RS03275 (MRBBS_0696) pilQ 726081..728198 (+) 2118 WP_014869997.1 type IV pilus secretin PilQ Machinery gene
  MRBBS_RS03280 (MRBBS_0697) aroK 728338..728904 (+) 567 WP_014869998.1 shikimate kinase AroK -
  MRBBS_RS03285 (MRBBS_0698) aroB 728897..729988 (+) 1092 WP_014869999.1 3-dehydroquinate synthase -
  MRBBS_RS03290 (MRBBS_0699) - 730014..731612 (+) 1599 WP_014870000.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75734.31 Da        Isoelectric Point: 4.3638

>NTDB_id=52311 MRBBS_RS03275 WP_014869997.1 726081..728198(+) (pilQ) [Marinobacter sp. BSs20148]
MNNSNMNAQKCLKPRLAMFKKFNVIVCAVTIGLLSSLANAATLQDVSFSSLPGDRLEVILQFDGPPPEPTGYTIDRPARI
AVDLKDTTSALGARSLPLGSGNAQSMTVVETNDRTRLIFNLIELVPYTTQRNNNALVMIIGGDFADVASNVAESDGASPV
ASSADAAPELVDVDFRRGAQGEGRVVVRLASATTQVDLSELGGKIRLTMPDLTVPERLRRRLDVTDFATPVQRIDTFIEG
GSAVIEIRPEGDYDYIAYQSGNEFTVSVERLSEEEAETRREEKFPYSGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDT
VGGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPADEIAARERLELETTKQIAELAPVRLDIIQVNYAKAAEVV
ALVQADQELISSRGFISSDDRTNTISVRETIEKLDEIRRLVSTWDVPVRQVSIEARIVRAQTNVAESLGVRWGGASFGNN
GNNSTVVGGSLDTVGSVMGAGAANSTSVTFPGALAVDLGVSGDGASSFAVGFSGGDFLVDLELSALESDGKAEIVSQPRV
VTADRQTASIKSGQEIPYQEASSSGATSTSFKEAVLSLEVTPQITPDDKIIMDLEVTQDSRGEDTPSGPAINTNSVTTQV
LVANGETIVLGGIFESTNTETTTKTPFLGDIPYLGRLFKRTQTTELRSELLIFITPKIIKNDLVR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=52311 MRBBS_RS03275 WP_014869997.1 726081..728198(+) (pilQ) [Marinobacter sp. BSs20148]
ATGAATAATAGCAACATGAATGCACAAAAATGCTTGAAGCCGAGGCTAGCGATGTTCAAAAAATTCAACGTAATTGTCTG
CGCGGTAACCATTGGGTTGCTGTCCAGCCTTGCTAACGCTGCCACTTTGCAGGATGTGTCGTTTTCCTCTTTACCCGGGG
ATCGGCTGGAAGTGATTTTGCAGTTTGACGGCCCACCGCCTGAACCTACGGGTTACACCATTGATCGCCCCGCTCGTATT
GCGGTAGACCTGAAAGACACCACCAGTGCCCTAGGTGCCCGCAGCTTGCCGCTCGGCAGCGGGAATGCGCAAAGCATGAC
CGTTGTTGAAACTAACGACCGTACCCGTCTTATTTTCAACCTGATTGAGCTGGTTCCTTACACCACTCAACGCAATAACA
ACGCGTTGGTGATGATTATTGGCGGCGATTTCGCAGATGTAGCCAGTAACGTTGCGGAATCTGACGGTGCCAGCCCTGTT
GCCTCGTCAGCGGACGCAGCGCCAGAGTTAGTAGACGTCGATTTTCGCCGCGGTGCACAAGGTGAAGGCCGTGTGGTTGT
GCGTCTGGCCAGTGCGACAACTCAAGTTGACCTGAGCGAGCTGGGCGGAAAAATTCGACTGACCATGCCGGATTTGACTG
TGCCCGAGCGCCTACGCCGCCGTTTGGACGTGACAGATTTTGCCACGCCGGTACAGCGGATCGATACTTTTATTGAGGGG
GGCAGTGCGGTTATAGAAATTCGTCCGGAAGGCGACTATGACTACATTGCGTACCAGTCCGGCAACGAATTTACTGTGAG
TGTTGAACGGCTAAGCGAAGAAGAAGCAGAAACTCGGCGCGAAGAGAAGTTCCCATATTCCGGCGACAAGCTCTCGCTAA
ATTTTCAAGACATTGAAGTTCGGTCGGTTCTTCAGCTGATCGCCGACTTTACCGGTTTGAATCTGGTGGCCAGCGATACC
GTTGGTGGTAGCATCACGCTGCGCTTGCAGAATGTGCCTTGGGATCAGGCGCTGGATCTGATTTTGAAAACCAAAGGTTT
GGATAAGCGCCAGATTGGCAATGTATTACTGGTAGCGCCCGCCGATGAAATCGCCGCCCGTGAACGGCTGGAACTGGAAA
CAACCAAGCAGATCGCCGAGCTTGCCCCGGTGCGTTTGGACATCATTCAGGTCAATTACGCAAAAGCTGCCGAAGTAGTA
GCGCTGGTGCAGGCTGACCAAGAGCTGATTTCTAGCCGCGGTTTCATTTCCTCAGACGACCGAACCAACACCATCAGTGT
GCGTGAAACCATTGAAAAACTGGACGAGATCCGTCGCCTTGTGTCGACATGGGACGTACCCGTTCGTCAGGTTTCCATTG
AAGCTCGGATTGTGCGCGCGCAAACCAACGTGGCCGAGAGCTTGGGCGTTCGCTGGGGCGGTGCATCTTTTGGTAATAAT
GGTAATAACTCGACGGTTGTAGGTGGTTCTCTTGATACGGTTGGTAGCGTAATGGGTGCCGGCGCTGCGAATTCAACCTC
GGTAACCTTTCCGGGTGCATTGGCGGTAGATCTTGGCGTATCTGGCGACGGCGCCTCATCCTTTGCCGTGGGCTTCAGCG
GCGGCGACTTCCTGGTAGACCTTGAACTGTCTGCGCTGGAAAGTGATGGCAAAGCGGAAATTGTGTCCCAGCCCAGAGTG
GTTACCGCAGATCGTCAGACGGCGTCTATCAAATCGGGTCAGGAAATCCCGTATCAGGAAGCGAGCAGTAGCGGCGCCAC
AAGCACTTCTTTTAAAGAAGCGGTGTTGTCATTGGAGGTAACGCCGCAGATCACACCGGATGACAAAATCATCATGGATC
TTGAAGTGACTCAGGACTCCCGTGGCGAAGACACCCCCAGCGGCCCGGCCATCAACACCAACTCGGTGACAACTCAGGTG
CTGGTCGCCAACGGTGAAACCATCGTTTTGGGCGGTATTTTCGAATCGACCAACACTGAAACCACTACCAAGACACCGTT
CTTGGGCGATATTCCCTACCTGGGGAGGTTGTTCAAGCGCACTCAGACTACAGAGCTGCGTAGCGAACTGCTGATTTTCA
TCACTCCGAAAATCATCAAAAACGACCTCGTTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(388-450)

Secretin and TonB N terminus short domain (STN)

(314-360)

Bacterial type II and III secretion system protein (Secretin)

(545-700)

AMIN domain (AMIN)

(54-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1FCB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

51.304

97.872

0.502
  comQ Acinetobacter baylyi ADP1

42.318

100

0.43
  pilQ Acinetobacter baumannii D1279779

40.766

100

0.423
  pilQ Acinetobacter baumannii strain A118

40.766

100

0.423
  pilQ Legionella pneumophila strain ERS1305867

40.327

95.319

0.384
  pilQ Legionella pneumophila strain Lp02

40.179

95.319

0.383

Multiple sequence alignment