Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   K5T46_RS18445 Genome accession   NZ_CP081489
Coordinates   3843143..3844267 (+) Length   374 a.a.
NCBI ID   WP_000228551.1    Uniprot ID   P30852
Organism   Escherichia coli BL21(DE3)     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3838143..3849267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5T46_RS18420 (K5T46_18420) mscL 3838269..3838679 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  K5T46_RS18425 (K5T46_18425) trkA 3838809..3840185 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  K5T46_RS18430 (K5T46_18430) rsmB 3840207..3841496 (-) 1290 WP_000744778.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  K5T46_RS18435 (K5T46_18435) fmt 3841542..3842489 (-) 948 WP_000004473.1 methionyl-tRNA formyltransferase -
  K5T46_RS18440 (K5T46_18440) def 3842504..3843013 (-) 510 WP_000114984.1 peptide deformylase -
  K5T46_RS18445 (K5T46_18445) dprA 3843143..3844267 (+) 1125 WP_000228551.1 DNA-protecting protein DprA Machinery gene
  K5T46_RS18450 (K5T46_18450) smg 3844239..3844712 (+) 474 WP_000460680.1 DUF494 family protein Smg -
  K5T46_RS18455 (K5T46_18455) yrdD 3844741..3845283 (+) 543 WP_001129722.1 type I DNA topoisomerase -
  K5T46_RS18460 (K5T46_18460) tsaC 3845288..3845860 (+) 573 WP_001301412.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  K5T46_RS18465 (K5T46_18465) aroE 3845865..3846683 (+) 819 WP_000451243.1 shikimate dehydrogenase -
  K5T46_RS18470 (K5T46_18470) yrdB 3846680..3846937 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  K5T46_RS18475 (K5T46_18475) yrdA 3846913..3847467 (-) 555 WP_001286158.1 gamma carbonic anhydrase family protein -
  K5T46_RS18480 (K5T46_18480) cybB 3847988..3848518 (+) 531 WP_000428998.1 cytochrome b561 -
  K5T46_RS18485 (K5T46_18485) - 3848763..3848936 (+) 174 WP_000731833.1 periplasmic protein -
  K5T46_RS18490 (K5T46_18490) yncP 3848962..3849030 (+) 69 WP_211180495.1 protein YncP -
  K5T46_RS18495 (K5T46_18495) hokB 3849008..3849157 (-) 150 WP_001320773.1 type I toxin-antitoxin system toxin HokB -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=522776 K5T46_RS18445 WP_000228551.1 3843143..3844267(+) (dprA) [Escherichia coli BL21(DE3)]
MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=522776 K5T46_RS18445 WP_000228551.1 3843143..3844267(+) (dprA) [Escherichia coli BL21(DE3)]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.238

98.663

0.476
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

45.104

90.107

0.406
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39