Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   I5P57_RS22640 Genome accession   NZ_CP066740
Coordinates   4713050..4714570 (+) Length   506 a.a.
NCBI ID   WP_001765502.1    Uniprot ID   A0A3K4FZM5
Organism   Escherichia coli strain R12     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4708050..4719570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5P57_RS22615 (I5P57_04425) ilvE 4709604..4710533 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  I5P57_RS22620 (I5P57_04426) ilvM 4710553..4710816 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  I5P57_RS22625 (I5P57_04427) ilvG 4710813..4712459 (-) 1647 WP_001012593.1 acetolactate synthase 2 catalytic subunit -
  I5P57_RS22630 ilvX 4712462..4712512 (-) 51 WP_001387183.1 peptide IlvX -
  I5P57_RS22635 ilvL 4712599..4712697 (-) 99 WP_001311244.1 ilv operon leader peptide -
  I5P57_RS22640 (I5P57_04428) comM 4713050..4714570 (+) 1521 WP_001765502.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  I5P57_RS22645 (I5P57_04429) maoP 4714595..4714933 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  I5P57_RS22650 (I5P57_04430) hdfR 4715052..4715891 (+) 840 WP_001765503.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55297.43 Da        Isoelectric Point: 7.4104

>NTDB_id=522235 I5P57_RS22640 WP_001765502.1 4713050..4714570(+) (comM) [Escherichia coli strain R12]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVMPG
ENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=522235 I5P57_RS22640 WP_001765502.1 4713050..4714570(+) (comM) [Escherichia coli strain R12]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCAGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTACTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAACGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCAATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCCCCCGGCATTTTGAGTAAAACGGTAATGCCGGGA
GAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAAAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3K4FZM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.317

100

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio cholerae strain A1552

58.765

99.209

0.583
  comM Vibrio campbellii strain DS40M4

57.769

99.209

0.573
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441