NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	JFJ84_RS01380	Genome accession	NZ_CP066739
Coordinates	269555..270013 (+)	Length	152 a.a.
NCBI ID	WP_012340401.1	Uniprot ID	-
Organism	Histophilus somni strain ASc-MMNZ-VFA-069
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 264555..275013

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JFJ84_RS01350 (JFJ84_01345)	ispB	264576..265547 (-)	972	WP_041604961.1	octaprenyl diphosphate synthase	-
JFJ84_RS01355 (JFJ84_01350)	rplU	265788..266099 (+)	312	WP_011608399.1	50S ribosomal protein L21	-
JFJ84_RS01360 (JFJ84_01355)	rpmA	266120..266377 (+)	258	WP_011608400.1	50S ribosomal protein L27	-
JFJ84_RS01365 (JFJ84_01360)	-	266513..267433 (+)	921	WP_012340377.1	DMT family transporter	-
JFJ84_RS01370 (JFJ84_01365)	cgtA	267443..268621 (+)	1179	WP_012340385.1	Obg family GTPase CgtA	-
JFJ84_RS01375 (JFJ84_01370)	ampD	268863..269432 (-)	570	WP_012340392.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
JFJ84_RS01380 (JFJ84_01375)	pilA	269555..270013 (+)	459	WP_012340401.1	prepilin peptidase-dependent pilin	Machinery gene
JFJ84_RS01385 (JFJ84_01380)	artP	270159..270896 (+)	738	WP_012340409.1	arginine ABC transporter ATP-binding protein ArtP	-
JFJ84_RS01390 (JFJ84_01385)	-	270914..271639 (+)	726	WP_012340415.1	transporter substrate-binding domain-containing protein	-
JFJ84_RS01395 (JFJ84_01390)	artQ	271644..272315 (+)	672	WP_011608407.1	arginine ABC transporter permease ArtQ	-
JFJ84_RS01400 (JFJ84_01395)	artM	272326..273009 (+)	684	WP_011608408.1	arginine ABC transporter permease ArtM	-
JFJ84_RS01405 (JFJ84_01400)	-	273115..273372 (-)	258	WP_178384284.1	DUF5339 domain-containing protein	-

Sequence

Protein

Download Length: 152 a.a. Molecular weight: 15903.18 Da Isoelectric Point: 8.4638

                                >NTDB_id=522194 JFJ84_RS01380 WP_012340401.1 269555..270013(+) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-069]

MKITYIFSSVKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITELLQASAPYRAEVELCVYNNDAPTNCNAGTNGI

QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide

Download Length: 459 bp

                                >NTDB_id=522194 JFJ84_RS01380 WP_012340401.1 269555..270013(+) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-069]

ATGAAAATAACCTATATTTTTTCATCAGTTAAAAAAGGATTTACTCTCATAGAACTGATGATTGTTATTGCTATTGTGGC

AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGCTATTGCAAGCTTCAG

CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT

CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG

AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA

ATAAGGCGGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-142)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	65.306	96.711	0.632
pilA	Haemophilus influenzae Rd KW20	63.265	96.711	0.612
pilA	Glaesserella parasuis strain SC1401	50.323	100	0.513
pilA	Ralstonia pseudosolanacearum GMI1000	35.714	100	0.395
pilA	Vibrio campbellii strain DS40M4	38.356	96.053	0.368

Detailed information NTG0522194