Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   JCP82_RS01380 Genome accession   NZ_CP066673
Coordinates   278090..279379 (-) Length   429 a.a.
NCBI ID   WP_002290101.1    Uniprot ID   A0A828ZUK2
Organism   Enterococcus faecium strain Dallas 163_1     
Function   repress competence development (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 264861..322681 278090..279379 within 0


Gene organization within MGE regions


Location: 264861..322681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JCP82_RS01310 (JCP82_01310) - 265025..265711 (-) 687 WP_002289316.1 GntR family transcriptional regulator -
  JCP82_RS01315 (JCP82_01315) - 265847..266041 (-) 195 WP_002315615.1 hypothetical protein -
  JCP82_RS01320 (JCP82_01320) - 266091..266531 (-) 441 WP_002301667.1 acyl-CoA thioesterase -
  JCP82_RS01325 (JCP82_01325) - 266535..267380 (-) 846 WP_002299303.1 NAD(P)H-hydrate dehydratase -
  JCP82_RS01330 (JCP82_01330) pepV 267529..268947 (+) 1419 WP_002294157.1 dipeptidase PepV -
  JCP82_RS01335 (JCP82_01335) - 269045..270340 (-) 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
  JCP82_RS01340 (JCP82_01340) - 270545..271747 (-) 1203 WP_002299302.1 hypothetical protein -
  JCP82_RS01345 (JCP82_01345) - 271734..273521 (-) 1788 WP_002299301.1 DUF6020 family protein -
  JCP82_RS01350 (JCP82_01350) - 273534..274487 (-) 954 WP_002299300.1 glycosyltransferase family 2 protein -
  JCP82_RS01355 (JCP82_01355) - 274672..275022 (-) 351 WP_002309710.1 PepSY domain-containing protein -
  JCP82_RS01360 (JCP82_01360) pepA 275222..276301 (+) 1080 WP_002294156.1 glutamyl aminopeptidase -
  JCP82_RS01365 (JCP82_01365) - 276444..276764 (+) 321 WP_002292632.1 thioredoxin family protein -
  JCP82_RS01370 (JCP82_01370) - 276786..277250 (+) 465 WP_002345012.1 universal stress protein -
  JCP82_RS01375 (JCP82_01375) ytpR 277446..278051 (+) 606 WP_002313997.1 YtpR family tRNA-binding protein -
  JCP82_RS01380 (JCP82_01380) htrA 278090..279379 (-) 1290 WP_002290101.1 S1C family serine protease Regulator
  JCP82_RS01385 (JCP82_01385) rlmH 279807..280286 (+) 480 WP_002345011.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  JCP82_RS01390 (JCP82_01390) - 280639..280953 (+) 315 WP_000420682.1 YdcP family protein -
  JCP82_RS01395 (JCP82_01395) - 280969..281355 (+) 387 WP_000985015.1 YdcP family protein -
  JCP82_RS01400 (JCP82_01400) - 281384..282769 (+) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  JCP82_RS01405 (JCP82_01405) - 282772..282925 (+) 154 Protein_280 conjugal transfer protein -
  JCP82_RS01410 (JCP82_01410) mobT 282948..284153 (+) 1206 WP_000398284.1 MobT family relaxase -
  JCP82_RS01415 (JCP82_01415) ltrA 284937..286847 (+) 1911 WP_002286940.1 group II intron reverse transcriptase/maturase -
  JCP82_RS01420 (JCP82_01420) - 286951..287175 (+) 225 WP_032509114.1 hypothetical protein -
  JCP82_RS01425 (JCP82_01425) - 287292..287789 (+) 498 WP_002345010.1 antirestriction protein ArdA -
  JCP82_RS01430 (JCP82_01430) - 287876..288268 (+) 393 WP_002345009.1 conjugal transfer protein -
  JCP82_RS01435 (JCP82_01435) - 288252..290699 (+) 2448 WP_000331148.1 ATP-binding protein -
  JCP82_RS01440 (JCP82_01440) - 290702..292885 (+) 2184 WP_002345008.1 CD3337/EF1877 family mobilome membrane protein -
  JCP82_RS01445 (JCP82_01445) - 292882..293883 (+) 1002 WP_002345007.1 lysozyme family protein -
  JCP82_RS01450 (JCP82_01450) - 293898..294812 (+) 915 WP_002345006.1 conjugal transfer protein -
  JCP82_RS15660 - 295126..295173 (+) 48 Protein_290 hypothetical protein -
  JCP82_RS01460 (JCP82_01460) tet(M) 295189..297108 (+) 1920 WP_002345005.1 tetracycline resistance ribosomal protection protein Tet(M) -
  JCP82_RS01465 (JCP82_01465) tet(L) 297206..298579 (+) 1374 WP_002345004.1 tetracycline efflux MFS transporter Tet(L) -
  JCP82_RS01470 (JCP82_01470) mobV 299143..300405 (+) 1263 WP_000119405.1 MobV family relaxase -
  JCP82_RS01475 (JCP82_01475) - 300650..301537 (+) 888 WP_025479406.1 protein rep -
  JCP82_RS01480 (JCP82_01480) - 301560..302240 (-) 681 WP_001015311.1 IS6-like element IS1216 family transposase -
  JCP82_RS01485 (JCP82_01485) - 302992..303726 (-) 735 WP_002345002.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(T) -
  JCP82_RS01490 (JCP82_01490) - 303793..304971 (-) 1179 WP_000997695.1 IS256-like element ISEf1 family transposase -
  JCP82_RS01495 (JCP82_01495) ermCL 305120..305179 (-) 60 WP_002503927.1 23S rRNA methylase leader peptide ErmCL -
  JCP82_RS01500 (JCP82_01500) - 305281..305961 (-) 681 WP_001015311.1 IS6-like element IS1216 family transposase -
  JCP82_RS01505 (JCP82_01505) - 306168..306845 (+) 678 WP_025477866.1 DUF4767 domain-containing protein -
  JCP82_RS01510 (JCP82_01510) sstT 307187..308407 (+) 1221 WP_002352746.1 serine/threonine transporter SstT -
  JCP82_RS01515 (JCP82_01515) - 308554..309189 (+) 636 WP_002294730.1 DUF998 domain-containing protein -
  JCP82_RS01520 (JCP82_01520) truA 309380..310123 (+) 744 WP_002300898.1 tRNA pseudouridine(38-40) synthase TruA -
  JCP82_RS01525 (JCP82_01525) msrA 310357..311190 (+) 834 WP_002300896.1 peptide-methionine (S)-S-oxide reductase MsrA -
  JCP82_RS01530 (JCP82_01530) - 311304..312264 (+) 961 Protein_305 aldo/keto reductase -
  JCP82_RS01535 (JCP82_01535) - 312553..313251 (+) 699 WP_002300890.1 FadR/GntR family transcriptional regulator -
  JCP82_RS01540 (JCP82_01540) - 313401..313877 (+) 477 Protein_307 PTS sugar transporter subunit IIC -
  JCP82_RS01545 (JCP82_01545) - 313937..315063 (+) 1127 WP_098381422.1 IS3 family transposase -
  JCP82_RS01550 (JCP82_01550) - 315102..316064 (+) 963 Protein_309 glycoside hydrolase family 1 protein -
  JCP82_RS01555 (JCP82_01555) - 316330..317625 (-) 1296 WP_002297185.1 ISL3-like element ISEfa5 family transposase -
  JCP82_RS01560 (JCP82_01560) - 317987..318307 (+) 321 WP_002301150.1 PTS sugar transporter subunit IIB -
  JCP82_RS01565 (JCP82_01565) - 318333..318650 (+) 318 WP_002287579.1 PTS lactose/cellobiose transporter subunit IIA -
  JCP82_RS01570 (JCP82_01570) - 318679..319928 (+) 1250 Protein_313 PTS sugar transporter subunit IIC -
  JCP82_RS01575 (JCP82_01575) - 320057..320521 (+) 465 WP_002299899.1 GNAT family N-acetyltransferase -
  JCP82_RS01580 (JCP82_01580) - 320550..320870 (-) 321 WP_002287574.1 YrhK family protein -
  JCP82_RS01585 (JCP82_01585) - 320901..322034 (-) 1134 WP_002301143.1 alcohol dehydrogenase catalytic domain-containing protein -
  JCP82_RS15280 - 322412..322612 (-) 201 WP_174236399.1 hypothetical protein -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 44916.93 Da        Isoelectric Point: 4.5419

>NTDB_id=521586 JCP82_RS01380 WP_002290101.1 278090..279379(-) (htrA) [Enterococcus faecium strain Dallas 163_1]
MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAMGTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKA
VDKVQDAVVSVINLQSQNQSSGFGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANSVTSGIISSLNRQVTSTNESNE
TVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALG
ITMVDLSAVSTQQQEQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKVGDSVKIT
YYEGSKEKTTTVELTIDQSALKQNQNSEN

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=521586 JCP82_RS01380 WP_002290101.1 278090..279379(-) (htrA) [Enterococcus faecium strain Dallas 163_1]
ATGGATAGAAAAAATGTGACACCAAAGATGAAAAAAAATAAAAATAATAGCCTCTGGCGTAAGTTGGGTCTGGGGCTTGT
TGGAGGGATCGTTGGAGGACTACTGACAGCTGGCATTTTTTATGCAGCGATGGGCACTGGCAACAATAGTTCTACTGCTA
CAAGCGGCAATCAAAATTCTGCCGGTGAAACAGTTGTGGAAAATGTCAAAGTCAATGTAGATTCTGATATCACCAAAGCG
GTAGATAAAGTTCAAGACGCTGTCGTTTCTGTTATCAACTTACAAAGTCAAAACCAATCAAGCGGTTTTGGCGGATTATT
CGGGCAGCAAGAAGAAAGTAGCAGCTCAGATGATAGTAATTTGGAAGCTTATAGTGAAGGAAGCGGCGTAATCTACAAAA
AAGACGGGAATACTGCTTATGTAGTCACAAACAATCACGTAGTAGATGGACAGCAAGGTTTGGAAGTCATGATGAAAGAC
GGTACGAAGGTAAAAGCTGAATTAGTTGGAACAGATGCCTATTCAGATTTAGCGGTGTTGAAGATCAATTCAGACAAAGT
CGAAACGGTTGCCTCTTTTGGGGACTCAAGTGCTTTGAAGGTTGGTGAACCTGCGATTGCGATTGGTTCCCCATTAGGAT
CTGAATATGCGAATTCTGTCACTTCAGGAATCATTTCTTCCTTGAACCGACAAGTAACAAGTACGAATGAATCGAACGAA
ACAGTCAATATCAATGCGATCCAAACAGATGCAGCGATCAACCCGGGAAATTCTGGTGGTCCTTTAGTGAATATCGAAGG
ACAAGTTATCGGTATCAACTCAAGTAAGATCGCAAGTACTTCTGAATCGACTTCTAATGTAAGCGTAGAAGGAATGGGAT
TTGCCATCCCAAGTAACGATGTAGTGAATATCATCAACCAACTGGAAAAAGACGGAAAAGTGACACGTCCAGCTCTAGGT
ATCACAATGGTCGACTTATCTGCCGTTTCTACTCAGCAACAAGAACAAATCTTGAAGATTCCTTCATCTGTAACTAACGG
TGTCATCGTGACAAATGTCCAAACAGCTACACCGGCAGAAAAAGCTGGGTTGAAACAATACGACGTCATTACTAAGATCG
ATGACAAAGAAGTTTCTTCTGGTGTAGAACTTCAATCTGCTTTGTATAAGAAAAAAGTGGGAGATTCTGTCAAAATCACT
TACTATGAAGGCTCGAAAGAAAAAACAACTACTGTTGAATTGACGATCGATCAATCGGCATTAAAACAAAATCAAAATAG
TGAAAATTAA

Domains

Predicted by InterproScan.

Peptidase family M50 (Peptidase_M50)

(10-417)

Trypsin-like peptidase domain (Trypsin_2)

(127-272)

PDZ domain (PDZ_2)

(317-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A828ZUK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

54.569

91.841

0.501
  htrA Streptococcus mutans UA159

60.169

82.517

0.496
  htrA Streptococcus mitis NCTC 12261

52.778

92.308

0.487
  htrA Streptococcus pneumoniae TIGR4

59.05

78.555

0.464
  htrA Streptococcus pneumoniae Rx1

59.05

78.555

0.464
  htrA Streptococcus pneumoniae D39

59.05

78.555

0.464
  htrA Streptococcus pneumoniae R6

59.05

78.555

0.464