Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LPO_RS04750 Genome accession   NC_018139
Coordinates   1031868..1032932 (+) Length   354 a.a.
NCBI ID   WP_014841276.1    Uniprot ID   A0AAV2UWB6
Organism   Legionella pneumophila subsp. pneumophila     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1026868..1037932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPO_RS04740 (LPO_1009) - 1027908..1030292 (-) 2385 WP_014841274.1 penicillin-binding protein 1A -
  LPO_RS04745 (LPO_1010) ravI 1030444..1031451 (-) 1008 WP_014841275.1 Dot/Icm T4SS effector RavI -
  LPO_RS04750 (LPO_1011) pilM 1031868..1032932 (+) 1065 WP_014841276.1 type IV pilus assembly protein PilM Machinery gene
  LPO_RS04755 (LPO_1012) pilN 1032936..1033484 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  LPO_RS04760 (LPO_1013) pilO 1033498..1034097 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  LPO_RS04765 (LPO_1014) pilP 1034094..1034678 (+) 585 WP_014841278.1 pilus assembly protein PilP Machinery gene
  LPO_RS04770 (LPO_1015) pilQ 1034682..1036778 (+) 2097 WP_014841279.1 type IV pilus secretin PilQ Machinery gene
  LPO_RS04775 (LPO_1016) aroK 1037224..1037751 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38727.73 Da        Isoelectric Point: 4.9540

>NTDB_id=52120 LPO_RS04750 WP_014841276.1 1031868..1032932(+) (pilM) [Legionella pneumophila subsp. pneumophila]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSASLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EEAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=52120 LPO_RS04750 WP_014841276.1 1031868..1032932(+) (pilM) [Legionella pneumophila subsp. pneumophila]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATAA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACTTCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAAGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCGCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGACAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAGCTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGGAGGCTATTGCCCTAAAAAATCAGGGTAAGTTACCAGAGGACTATGAATCCGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.153

100

0.992
  comM Acinetobacter nosocomialis M2

43.38

100

0.435
  pilM Acinetobacter baumannii D1279779

43.099

100

0.432
  comM Acinetobacter baylyi ADP1

41.737

100

0.421

Multiple sequence alignment