Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JFL57_RS08250 Genome accession   NZ_CP066565
Coordinates   1815893..1817308 (-) Length   471 a.a.
NCBI ID   WP_233125748.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-076     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1810893..1822308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFL57_RS08235 (JFL57_08235) ffh 1812207..1813583 (-) 1377 WP_198986889.1 signal recognition particle protein -
  JFL57_RS08240 (JFL57_08240) - 1813733..1814530 (+) 798 WP_075293627.1 cytochrome C assembly family protein -
  JFL57_RS08245 (JFL57_08245) - 1814600..1815859 (+) 1260 WP_012341088.1 HlyC/CorC family transporter -
  JFL57_RS08250 (JFL57_08250) pilB 1815893..1817308 (-) 1416 WP_233125748.1 GspE/PulE family protein Machinery gene
  JFL57_RS08255 (JFL57_08255) tig 1817667..1818965 (+) 1299 WP_198986890.1 trigger factor -
  JFL57_RS08260 (JFL57_08260) clpP 1819051..1819632 (+) 582 WP_075293629.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  JFL57_RS08265 (JFL57_08265) clpX 1819648..1820892 (+) 1245 WP_198986891.1 ATP-dependent protease ATP-binding subunit ClpX -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 53824.51 Da        Isoelectric Point: 5.9894

>NTDB_id=520982 JFL57_RS08250 WP_233125748.1 1815893..1817308(-) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-076]
MIIPQSEDMSPIVMSIDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPV
LLNNQLLKQLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQNVSDIHIEPQMNCLQIRFR
IDGILQCQSPLPFSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDXRLSTLPTNMGEKAVLRLQQNKPVQLS
FAELGMTENQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDF
SRLLRTFLRQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRK
KCPHTENENCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSSE

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=520982 JFL57_RS08250 WP_233125748.1 1815893..1817308(-) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-076]
ATGATAATTCCTCAATCTGAAGATATGTCTCCGATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACCGCATTT
ATGGCAAAGAAATCAGCAACAATCCCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTAT
GGTTAGGCGTTGATAGTCTAAATAATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTT
TTACTTAATAACCAATTACTTAAACAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACC
TATAAATCAATATGTAGCCGAGCATCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACA
TTTTTGAAAATGCGTTAAAGCAAAATGTATCAGATATTCATATTGAACCACAAATGAATTGCTTGCAAATACGTTTCAGA
ATAGACGGTATTTTACAATGCCAATCGCCTCTTCCGTTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGC
CAAATTGGATATAAGCGAAACACGGTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATW
TTCGTTTATCAACCCTTCCGACGAATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGT
TTTGCGGAACTCGGTATGACAGAAAACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGT
GACAGGCCCAACAGGGAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTGAATGATAAGCACAAACATATTA
TGACAGCGGAAGATCCGATAGAAATTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTT
AGCCGACTGTTAAGAACTTTTCTTCGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAAT
AATGGCATTAAGAGCAGCACAAACCGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTCTCAC
GCTTGCTTCAATTGGGGGTAAAGCAACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAA
AAATGTCCACATACAGAAAATGAAAATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACA
ACCGCATCTAATAGATAATCATATTTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGT
TAGAAATAACAGATTTAGCAGAAGTGGATAGAGTTATTGGACAAAGTAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-98)

Type II/IV secretion system protein (T2SSE)

(134-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.33

97.877

0.669
  pilB Haemophilus influenzae Rd KW20

67.609

97.665

0.66
  pilB Glaesserella parasuis strain SC1401

57.333

95.541

0.548
  pilB Vibrio campbellii strain DS40M4

40.041

100

0.41
  pilB Vibrio cholerae strain A1552

39.212

100

0.401
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.401
  pilB Legionella pneumophila strain ERS1305867

36.842

100

0.386
  pilB Acinetobacter baylyi ADP1

35.729

100

0.38
  pilB Acinetobacter baumannii D1279779

37.06

100

0.38