Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JE032_RS06350 Genome accession   NZ_CP066546
Coordinates   1371092..1372144 (+) Length   350 a.a.
NCBI ID   WP_198919653.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1366092..1377144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JE032_RS06340 - 1367044..1368312 (+) 1269 WP_198919652.1 malic enzyme-like NAD(P)-binding protein -
  JE032_RS06345 - 1368440..1370875 (-) 2436 WP_164485464.1 penicillin-binding protein 1A -
  JE032_RS06350 pilM 1371092..1372144 (+) 1053 WP_198919653.1 pilus assembly protein PilM Machinery gene
  JE032_RS06355 - 1372144..1372710 (+) 567 WP_009046598.1 PilN domain-containing protein -
  JE032_RS06360 - 1372707..1373324 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  JE032_RS06365 - 1373321..1373848 (+) 528 WP_009046600.1 pilus assembly protein PilP -
  JE032_RS06370 pilQ 1373862..1375934 (+) 2073 WP_110175403.1 type IV pilus secretin PilQ Machinery gene
  JE032_RS06375 aroK 1375939..1376457 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37075.56 Da        Isoelectric Point: 5.1221

>NTDB_id=520748 JE032_RS06350 WP_198919653.1 1371092..1372144(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTALSVVHEGQIIYSREQLFGGHRLTEHIQRRYGLSKEEAS
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=520748 JE032_RS06350 WP_198919653.1 1371092..1372144(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCAGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCGCCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCGACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCAGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

51.705

100

0.52
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457