Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JE032_RS06370 Genome accession   NZ_CP066546
Coordinates   1373862..1375934 (+) Length   690 a.a.
NCBI ID   WP_110175403.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1368862..1380934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JE032_RS06350 pilM 1371092..1372144 (+) 1053 WP_198919653.1 pilus assembly protein PilM Machinery gene
  JE032_RS06355 - 1372144..1372710 (+) 567 WP_009046598.1 PilN domain-containing protein -
  JE032_RS06360 - 1372707..1373324 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  JE032_RS06365 - 1373321..1373848 (+) 528 WP_009046600.1 pilus assembly protein PilP -
  JE032_RS06370 pilQ 1373862..1375934 (+) 2073 WP_110175403.1 type IV pilus secretin PilQ Machinery gene
  JE032_RS06375 aroK 1375939..1376457 (+) 519 WP_009041824.1 shikimate kinase AroK -
  JE032_RS06380 aroB 1376605..1377705 (+) 1101 WP_028683777.1 3-dehydroquinate synthase -
  JE032_RS06385 - 1377716..1379305 (+) 1590 WP_016704448.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74655.78 Da        Isoelectric Point: 5.4337

>NTDB_id=520749 JE032_RS06370 WP_110175403.1 1373862..1375934(+) (pilQ) [Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGPRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATVAQSAPGQPAVASGRAKATASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=520749 JE032_RS06370 WP_110175403.1 1373862..1375934(+) (pilQ) [Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGACCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGAGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGTGGCGCAGAGTGCAC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCACGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGTCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACGCCGAGCG
CTCAGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGATCTCGAGCCGCTGCGGCGTGAACTGCTGC
AGGTCAACTATGCCAAGGCGGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCTGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATTGGTACGCCGGGCAGCACCAGCAGCAATTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAAACCGGTAACGGCGAAA
TCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCTTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTCATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.049

100

0.736
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.852

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.852

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37