Detailed information NTG0051777

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: A458_RS40435
• Genome accession: NC_018028
• Coordinates: 4005363..4006427 (-)
• Length: 354 a.a.
• NCBI ID: WP_014821722.1
• Uniprot ID: I4CXY9
• Organism: Stutzerimonas stutzeri CCUG 29243
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4000363..4011427

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A458_RS40410 (A458_18590)	aroK	4000898..4001443 (-)	546	WP_014821719.1	shikimate kinase AroK	-
A458_RS40415 (A458_18595)	pilQ	4001449..4003593 (-)	2145	WP_014821720.1	type IV pilus secretin PilQ	Machinery gene
A458_RS40420 (A458_18600)	pilP	4003647..4004174 (-)	528	WP_003301042.1	type 4a pilus biogenesis lipoprotein PilP	-
A458_RS40425 (A458_18605)	pilO	4004171..4004794 (-)	624	WP_003301043.1	type 4a pilus biogenesis protein PilO	-
A458_RS40430 (A458_18610)	pilN	4004791..4005363 (-)	573	WP_014821721.1	type 4a pilus biogenesis protein PilN	-
A458_RS40435 (A458_18615)	comM	4005363..4006427 (-)	1065	WP_014821722.1	pilus assembly protein PilM	Machinery gene
A458_RS40440 (A458_18620)	-	4006608..4009040 (+)	2433	WP_014821723.1	penicillin-binding protein 1A	-
A458_RS40445 (A458_18625)	-	4009104..4010372 (-)	1269	WP_014821724.1	malic enzyme-like NAD(P)-binding protein	-
A458_RS40450 (A458_18630)	-	4010522..4011316 (-)	795	WP_014821725.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37675.16 Da
• Isoelectric Point: 4.6880

>NTDB_id=51777 A458_RS40435 WP_014821722.1 4005363..4006427(-) (comM) [Stutzerimonas stutzeri CCUG 29243]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVEAYAVEPLPANAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKIIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRSPGRAEVLLAACRKENIEIREAALAL
AGLTAKVVDVEAYALERSYGLLAPQLGAAHGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASISGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=51777 A458_RS40435 WP_014821722.1 4005363..4006427(-) (comM) [Stutzerimonas stutzeri CCUG 29243]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCAGGTAGTCGCTACCGCGTCGAGGCGTATGCTGTCGAGCCGCTCCCGGCAAACGCCGTAGTTGAAAAGAACA
TCGCTGAGCTCGAGGGCGTCGGCCAGGCATTGCAGCGGGTGGTTGCCAAGGCCAAGACCGGATGCAAGTCGGCGGTAGTG
GCTGTTTCAGGTTCCGCGGTAATCACCAAGATCATCGAGATGGATGCTGGTTTGTCCGACGAAGAGCTCGAGAATCAGCT
GAAGATCGAGGCGGACCAGTACATTCCCTATCCGCTGGAAGAAGTGGCGATCGACTTCGAAGTCCAGGGGCCGGCTCCTC
GCTCTCCTGGCCGAGCAGAAGTCCTGCTCGCCGCTTGCCGCAAGGAAAACATCGAAATTCGTGAGGCAGCGCTTGCGCTG
GCGGGGCTCACTGCCAAGGTGGTTGACGTCGAAGCCTATGCGCTTGAGCGGTCATACGGGCTACTCGCTCCACAGTTGGG
TGCGGCGCATGGAGAGCTGACCGTCGCCGTGGTGGATATCGGTGCCACCATGACTACCCTCAGTGTTCTGCATAACGGTC
GTACTATTTATACCCGTGAACAGCTTTTTGGTGGGCGTCAGCTGACGGAGGAAATTCAGCGACGCTACGGCCTTTCACAA
GAAGAAGCTGGTCTAGCCAAAAAGCAAGGCGGCCTCCCAGACGACTACGACAGTGAGGTTTTGCTTCCGTTCAAAGAGGC
AGTTGTGCAGCAGGTTTCTCGCTCGTTGCAGTTCTTCTTTGCGGCAGGACAGTTCCATGATGTCGACTGCATCCTGTTAG
CGGGCGGGACGGCATCGATATCTGGGTTGGATCACATGATTCAGCAGAAGATCGGTACTCAGACTCTAGTTGCGAATCCA
TTCGCCGATATGGCTTTGAGCAGCAAAGTGAATGCTGGAGCGCTGGCTAGCGATGCGCCGGCCTTGATGATTGCTTGTGG
CCTGGCGCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: I4CXY9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	55.085	100	0.551
pilM	Acinetobacter baumannii D1279779	55.085	100	0.551
comM	Acinetobacter baylyi ADP1	52.825	100	0.528
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466