Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A458_RS40415 Genome accession   NC_018028
Coordinates   4001449..4003593 (-) Length   714 a.a.
NCBI ID   WP_014821720.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri CCUG 29243     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3996449..4008593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A458_RS40400 (A458_18580) - 3998162..3999760 (-) 1599 WP_014821717.1 AAA family ATPase -
  A458_RS40405 (A458_18585) aroB 3999771..4000874 (-) 1104 WP_014821718.1 3-dehydroquinate synthase -
  A458_RS40410 (A458_18590) aroK 4000898..4001443 (-) 546 WP_014821719.1 shikimate kinase AroK -
  A458_RS40415 (A458_18595) pilQ 4001449..4003593 (-) 2145 WP_014821720.1 type IV pilus secretin PilQ Machinery gene
  A458_RS40420 (A458_18600) pilP 4003647..4004174 (-) 528 WP_003301042.1 type 4a pilus biogenesis lipoprotein PilP -
  A458_RS40425 (A458_18605) pilO 4004171..4004794 (-) 624 WP_003301043.1 type 4a pilus biogenesis protein PilO -
  A458_RS40430 (A458_18610) pilN 4004791..4005363 (-) 573 WP_014821721.1 type 4a pilus biogenesis protein PilN -
  A458_RS40435 (A458_18615) comM 4005363..4006427 (-) 1065 WP_014821722.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 76742.93 Da        Isoelectric Point: 4.7188

>NTDB_id=51776 A458_RS40415 WP_014821720.1 4001449..4003593(-) (pilQ) [Stutzerimonas stutzeri CCUG 29243]
MNTTLSRLGISLLAAFISPALLAANLNALDVATLPGDRVELRFAFDEPVAAPRGYTLDQPARIALDLPGISNKLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLNTLVPYSTRVDGNNLFVVLGESSGAVRAPAVSAAPVAPVAAPAKAMAVGKAIENID
FRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVLFDKAQLPEALRVRLDVQDFATPVKFVDSSAQAGGASVAIEPTGRYD
YLAYQTDNKLTISIKPLTEDEAEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVSYAKAADIAQLFQSVTDNGGGAE
ERGSIAVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNAWGRDGNL
GVADDPDASGKPGDEDYIPSRQVGRFPGVREIPLNTPFVDMGAIGATSGFGLGFVTDNAILDLQLSAMEKTGNGEVVSQP
KVVTADKETAKILKGAEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGVPAIRKN
EVNAKVLVTDGETIVIGGVFSNTQSKSVDKVPLLGDLPFVGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=51776 A458_RS40415 WP_014821720.1 4001449..4003593(-) (pilQ) [Stutzerimonas stutzeri CCUG 29243]
ATGAACACTACCCTTTCACGCCTAGGTATCTCTTTGCTGGCGGCGTTTATTTCTCCAGCCCTGCTGGCGGCCAACCTGAA
CGCCCTCGATGTCGCTACGCTGCCTGGCGACCGTGTTGAATTGAGGTTTGCGTTCGACGAACCGGTGGCTGCACCGCGTG
GTTATACCCTCGATCAGCCTGCGCGGATCGCGCTCGACCTACCCGGCATATCCAATAAGCTCGGAGCGAAGAATCGCGAG
CTAGGAGTTGGCAATGCGCGGAGCGTGACCGTCGTTGAAGCCCAAGGCCGTACCCGACTGATCGTTAATCTCAATACTCT
GGTGCCCTACTCCACTCGCGTAGATGGTAACAATCTCTTTGTGGTACTGGGCGAAAGTTCGGGTGCTGTACGTGCCCCAG
CTGTTTCAGCTGCGCCCGTTGCTCCAGTGGCTGCGCCTGCCAAGGCGATGGCTGTAGGTAAGGCGATCGAGAACATCGAT
TTCCGTCGGGGTGAAGACGGTGCCGGAAACGTCGTTATTACGCTCTCTGACCCATCGGTAGGCTCGACCATTGAGGAGCA
GGGCGGAAAGATTCGTGTCCTCTTCGACAAAGCTCAACTGCCGGAGGCGCTGCGTGTTCGTCTTGATGTTCAGGATTTCG
CTACGCCCGTGAAATTTGTCGATTCGTCCGCTCAGGCCGGTGGAGCTAGTGTCGCCATCGAGCCGACCGGTCGATACGAT
TATCTGGCGTACCAGACAGACAACAAACTGACCATCAGCATCAAGCCTCTGACCGAGGATGAAGCCGAGAAGCGCAAGGC
TGAGCGCTTTGCTTATTCGGGCGAGAAACTCTCGCTCAACTTTCAGGATATCGACGTTCGGTCCGTACTGCAGCTGATCG
CTGATTTCACCGATCTGAACCTGGTGGCGAGCGATACCGTCGCTGGCAACATAACCCTGCGTCTGCAAAACGTGCCTTGG
GACCAGGCGCTGGATCTGGTGCTTAAAACCAAAGGTCTGGATAAGCGCCAGGTTGGTAACGTGCTGCTGGTCGCGCCTGC
CGACGAGATTGCTGCCCGTGAGCGTCAGGAGCTTGAGTCGCAGCGGCAGATCGCCGAGCTTGCTCCACTTCGTCGCGAGG
TCGTGCAGGTCAGTTATGCAAAGGCTGCAGATATTGCCCAGTTGTTTCAGTCGGTGACAGACAATGGAGGAGGCGCTGAA
GAGCGGGGATCTATTGCTGTCGATGACCGTACGAACAATATAATTGCTTATCAAACACAGGATAGGTTGGATGAGTTACG
CCGTATTGTGGCGCAGTTGGATATACCCGTGCGCCAGGTGATGATTGAGGCAAGGATAGTCGAAGCCAATGTCGATTATG
ACAAGAGCCTTGGCGTGCGTTGGGGAGGCAATCTTTGGGCTGGCGACAAGTGGAATGCTTGGGGGCGTGACGGGAATCTT
GGAGTGGCAGACGACCCGGATGCCTCTGGTAAGCCTGGTGATGAGGACTATATTCCTTCGCGTCAGGTTGGACGCTTTCC
TGGGGTTCGGGAAATTCCACTAAACACGCCATTTGTGGATATGGGTGCCATAGGCGCAACGTCGGGATTTGGGCTGGGCT
TCGTGACCGATAATGCCATTCTTGACCTGCAATTGTCAGCGATGGAGAAAACCGGCAACGGTGAAGTCGTTTCGCAGCCT
AAAGTGGTGACCGCTGATAAGGAAACGGCAAAGATTCTGAAGGGTGCGGAGGTTCCTTACCAAGAGGCGAGTTCCAGCGG
TGCAACTAGCACTTCTTTTAAAGAGGCAGCTCTTTCGCTGGAGGTGACTCCACAGATTACCCCGGATAACCGGATCATCA
TGGAGGTAAAGGTTACTAAGGACGAGCCTGACTTTTCTCAAGCTGCCAGCACCGGTGGTGTTCCAGCCATCCGTAAGAAC
GAAGTTAATGCGAAGGTCCTGGTGACTGATGGCGAAACGATTGTTATCGGCGGTGTCTTTTCCAATACGCAGAGCAAGTC
CGTGGATAAGGTTCCTTTGCTAGGTGATCTTCCCTTCGTAGGGCGTCTGTTCCGTCGTGATTTGGTGCAGGATCGAAAAT
CGGAGCTGCTTGTGTTCATCACGCCTCGGATCATGAATAATCAGGCGATTCAAGCCAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Bacterial type II and III secretion system protein (Secretin)

(547-705)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.49

100

0.738
  comQ Acinetobacter baylyi ADP1

41.292

99.72

0.412
  pilQ Acinetobacter baumannii strain A118

39.136

100

0.394
  pilQ Acinetobacter baumannii D1279779

39.136

100

0.394

Multiple sequence alignment