Detailed information NTG0051753

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: A458_RS23245
• Genome accession: NC_018028
• Coordinates: 311711..312856 (+)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas stutzeri CCUG 29243
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 306711..317856

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A458_RS23225 (A458_01460)	-	308386..308976 (-)	591	WP_014818744.1	YggT family protein	-
A458_RS23230 (A458_01465)	proC	309027..309851 (-)	825	WP_014818745.1	pyrroline-5-carboxylate reductase	-
A458_RS23235 (A458_01470)	-	309862..310551 (-)	690	WP_014818746.1	YggS family pyridoxal phosphate-dependent enzyme	-
A458_RS23240 (A458_01475)	pilT	310616..311650 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
A458_RS23245 (A458_01480)	pilU	311711..312856 (+)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A458_RS43880	-	312853..313689 (-)	837	Protein_292	TIGR03915 family putative DNA repair protein	-
A458_RS23255 (A458_01495)	-	313689..314900 (-)	1212	WP_014818748.1	putative DNA modification/repair radical SAM protein	-
A458_RS23260 (A458_01500)	-	315034..315429 (+)	396	WP_003282737.1	NINE protein	-
A458_RS23265 (A458_01505)	-	315507..316781 (-)	1275	WP_014818749.1	dihydroorotase	-
A458_RS23270 (A458_01510)	-	316781..317797 (-)	1017	WP_003303145.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=51753 A458_RS23245 WP_014818747.1 311711..312856(+) (pilU) [Stutzerimonas stutzeri CCUG 29243]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=51753 A458_RS23245 WP_014818747.1 311711..312856(+) (pilU) [Stutzerimonas stutzeri CCUG 29243]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCTCGTGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGGGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGACGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGATTTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCCCAGGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388