Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6H63_RS10150 Genome accession   NZ_CP066057
Coordinates   2108890..2110593 (-) Length   567 a.a.
NCBI ID   WP_066893592.1    Uniprot ID   A0A1B8QT22
Organism   Moraxella nonliquefaciens strain FDAARGOS_1006     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2103890..2115593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H63_RS10130 (I6H63_10135) - 2104733..2105248 (-) 516 WP_066886663.1 hypothetical protein -
  I6H63_RS10135 (I6H63_10140) metN 2106055..2107101 (+) 1047 WP_067006336.1 methionine ABC transporter ATP-binding protein MetN -
  I6H63_RS10140 (I6H63_10145) - 2107118..2107780 (+) 663 WP_228141017.1 methionine ABC transporter permease -
  I6H63_RS10145 (I6H63_10150) - 2107905..2108783 (+) 879 WP_067006333.1 MetQ/NlpA family lipoprotein -
  I6H63_RS10150 (I6H63_10155) pilB 2108890..2110593 (-) 1704 WP_066893592.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6H63_RS10155 (I6H63_10160) tpiA 2110849..2111628 (+) 780 WP_067006330.1 triose-phosphate isomerase -
  I6H63_RS10160 (I6H63_10165) secG 2111640..2111933 (+) 294 WP_066886650.1 preprotein translocase subunit SecG -
  I6H63_RS10165 (I6H63_10170) era 2112013..2113029 (-) 1017 WP_067006327.1 GTPase Era -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61950.15 Da        Isoelectric Point: 5.1376

>NTDB_id=516909 I6H63_RS10150 WP_066893592.1 2108890..2110593(-) (pilB) [Moraxella nonliquefaciens strain FDAARGOS_1006]
MSAPNFGGLAHILVSNELVAPDAMTKALTEAQQQNMAIVPYLVQNKVAPANKIAKLLAHEFGDPLFDLNALSADAVNKDL
VDEKIINQYGALPLFRRGNRLFVGLSDPTRLDAIDAISFNAKLTVETVVVEEDKLKKMIENVYSGGTGLGDLGDVDLSDD
VEGGTDDDEGTDANAGSGEDAPVVKFVNKMLIDAIRMGASDLHFEPYEKSYRVRFRVDGVLQVISRPPIQLAGKIAARLK
VMSQMDISERRAPQDGRIKLKISKTKAIDFRVNSCPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFLEALEKPQGM
LLITGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNPKVGLTFASALKAFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKSCKRPTNVPKQSLLEMGFTEE
DLANPDNVIYEPVGCSECREGYKGRVGIYEVMKIHPSISRIIMEDGNAIQIKDEAIKHGFRDLRRSGIMKVLQGTTSIQE
MYRVTSE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=516909 I6H63_RS10150 WP_066893592.1 2108890..2110593(-) (pilB) [Moraxella nonliquefaciens strain FDAARGOS_1006]
ATGTCTGCTCCTAATTTTGGTGGTTTGGCACACATTTTGGTGTCAAATGAACTGGTCGCCCCTGATGCCATGACCAAGGC
ACTGACCGAAGCCCAACAGCAAAATATGGCGATTGTGCCGTATTTGGTACAAAACAAAGTTGCCCCTGCCAATAAGATTG
CCAAATTGCTTGCTCATGAGTTTGGTGACCCATTATTTGACCTAAATGCACTCTCTGCCGATGCGGTCAATAAAGACCTG
GTAGATGAGAAAATCATCAATCAGTATGGGGCGTTACCACTATTTAGGCGAGGTAACAGACTTTTTGTGGGCTTGTCTGA
CCCTACACGTTTAGATGCCATTGATGCCATTTCATTTAATGCTAAGCTGACCGTTGAGACCGTCGTCGTCGAAGAAGATA
AGCTTAAAAAGATGATTGAAAATGTCTACTCAGGCGGCACGGGTTTGGGTGATTTGGGCGATGTGGATTTGTCGGATGAT
GTGGAGGGTGGCACAGACGATGATGAGGGGACAGATGCCAATGCTGGCAGCGGTGAGGATGCCCCTGTGGTCAAGTTTGT
CAATAAAATGCTCATAGATGCCATTCGCATGGGGGCATCGGATTTGCATTTTGAGCCGTATGAGAAATCCTACCGTGTGC
GTTTTCGTGTGGATGGTGTGTTACAGGTCATCAGTCGTCCGCCCATTCAGCTGGCAGGCAAAATCGCCGCACGCTTAAAA
GTCATGTCGCAAATGGACATCTCCGAACGCAGAGCCCCCCAAGATGGTCGTATCAAGCTAAAAATCTCCAAAACCAAAGC
CATTGATTTTCGTGTGAACTCATGTCCGACACTCTTTGGCGAGAAACTTGTATTGCGTATTCTTGACCCATCATCTGCCA
TGCTCGGTATTGATGCGTTGGGTTATGAGCCTGACCAAAAAGAGCTCTTCTTAGAAGCCCTAGAAAAACCACAAGGCATG
CTCCTAATCACAGGCCCAACAGGTTCAGGTAAAACCGTCTCGCTCTACACAGGTCTAAACATCCTAAACACGCCTGAGAC
CAACATATCCACCGCCGAAGACCCTGTGGAGATTAACTTAGAAGGGATTAACCAAGTCAACGTAAACCCCAAGGTTGGCT
TGACCTTTGCTTCTGCTCTAAAAGCCTTCCTACGTCAAGACCCTGACATCGTCATGGTCGGTGAGATTCGTGATTTGGAA
ACCGCTGAGATTGCCATTAAAGCTGCCCAAACTGGTCATATGGTGTTATCCACCCTACATACCAACTCCGCTCCTGAGAC
CTTAACTCGTCTTAGAAACATGGGCGTGGCATCTTTTAATATTGCCACATCGGTGAATCTGGTCATCGCCCAACGTCTGG
CACGTCGCTTGTGTAAGTCATGCAAAAGACCGACCAATGTCCCCAAGCAGAGTCTGTTAGAAATGGGCTTTACCGAAGAA
GACCTTGCCAACCCTGACAATGTCATCTATGAACCTGTGGGCTGTTCTGAATGTCGTGAGGGCTATAAAGGGCGTGTGGG
CATCTATGAGGTGATGAAAATCCATCCGAGCATTTCACGGATTATCATGGAAGATGGTAACGCCATCCAAATCAAAGACG
AAGCGATAAAACACGGCTTTCGTGATTTGAGACGTTCTGGCATCATGAAAGTCTTACAAGGCACAACAAGCATCCAAGAG
ATGTATCGGGTAACCTCTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B8QT22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.254

100

0.674
  pilB Acinetobacter baylyi ADP1

66.667

100

0.667
  pilB Legionella pneumophila strain ERS1305867

52.043

99.295

0.517
  pilF Neisseria gonorrhoeae MS11

52.057

98.589

0.513
  pilB Vibrio cholerae strain A1552

49.117

99.824

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

50.847

93.651

0.476
  pilB Vibrio campbellii strain DS40M4

50.851

93.298

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.743

100

0.377
  pilF Thermus thermophilus HB27

36.842

100

0.37