Detailed information NTG0051653

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: THIVI_RS13895
• Genome accession: NC_018012
• Coordinates: 3150391..3151641 (+)
• Length: 416 a.a.
• NCBI ID: WP_014779206.1
• Uniprot ID: I3YCQ6
• Organism: Thiocystis violascens DSM 198
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3145391..3156641

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THIVI_RS13885 (Thivi_2861)	-	3148239..3149705 (+)	1467	WP_014779204.1	glutamate synthase subunit beta	-
THIVI_RS13890 (Thivi_2862)	-	3149801..3150232 (+)	432	WP_014779205.1	DUF29 domain-containing protein	-
THIVI_RS13895 (Thivi_2863)	pilU	3150391..3151641 (+)	1251	WP_014779206.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
THIVI_RS13900 (Thivi_2864)	pilU	3151668..3152723 (+)	1056	WP_014779207.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
THIVI_RS13905 (Thivi_2865)	gspD	3152811..3155009 (-)	2199	WP_245537275.1	type II secretion system secretin GspD	-
THIVI_RS13910 (Thivi_2866)	-	3155055..3155642 (-)	588	WP_014779209.1	type II secretion system protein N	-
THIVI_RS13915 (Thivi_2867)	gspM	3155639..3156253 (-)	615	WP_014779210.1	type II secretion system protein GspM	-

Sequence

Protein

• Length: 416 a.a.
• Molecular weight: 45937.64 Da
• Isoelectric Point: 6.0951

>NTDB_id=51653 THIVI_RS13895 WP_014779206.1 3150391..3151641(+) (pilU) [Thiocystis violascens DSM 198]
MDRQQAAHFMLECLRKMIAKKGSDLFISAGFPPACKIDGRMTPMSSQILSGEQTNILVRSIMNDRQVRDFDAHKECNFAI
SPQDIGRFRVNAFVQQGLCGAVLRTINAGIPTLEELNLPHALKEIVMNKRGLVLLVGGTGSGKSTSLAAMLGHRNENSYG
HIITIEDPVEYVHPHRNCLITQREVGVDTESWDAALINTLRQAPDVILIGEIRTRETMEHAINFSETGHLCLSTLHANNA
NQALDRIINLFPEERRSQLLMDLSLNLNAVISQRLLPCTDGGRIAAVEVMINSPLIQDMIFKGDVGGIKSIMKKSRELGM
QTFDMSLFDLHEAGKINYEDALRNADSMNGLRLAIKLEGREARGKNHFAETDGLTMVGDEEEDVFDSTLFGEALPVSRIE
EPPAGLVNPAGSKARR

Nucleotide

• Length: 1251 bp

>NTDB_id=51653 THIVI_RS13895 WP_014779206.1 3150391..3151641(+) (pilU) [Thiocystis violascens DSM 198]
ATGGATCGTCAACAAGCCGCTCATTTCATGCTCGAATGTCTGCGCAAGATGATTGCTAAAAAAGGTTCCGACCTCTTCAT
CTCCGCCGGCTTTCCGCCCGCCTGCAAGATCGACGGCCGCATGACGCCCATGAGCAGCCAGATCCTGAGCGGCGAGCAGA
CCAATATCCTGGTGCGTTCCATCATGAACGACCGTCAGGTGCGCGACTTCGACGCGCACAAGGAATGCAATTTCGCGATC
AGCCCGCAGGATATCGGCCGTTTCCGGGTCAATGCCTTCGTGCAGCAGGGGCTGTGCGGCGCGGTGTTGCGGACGATCAA
CGCCGGGATTCCGACCCTGGAGGAGTTGAATCTGCCGCATGCGCTGAAAGAGATCGTGATGAACAAGCGCGGTCTGGTGC
TGCTGGTCGGCGGGACGGGATCCGGCAAATCGACCTCGCTGGCGGCCATGCTCGGTCATCGCAACGAAAATAGCTATGGC
CACATCATCACGATCGAGGATCCGGTCGAGTATGTGCATCCGCATCGGAACTGTCTCATCACCCAGCGCGAGGTTGGCGT
CGATACCGAGAGCTGGGACGCCGCGCTGATCAATACCCTGCGTCAGGCTCCGGACGTGATCCTGATCGGCGAGATCCGCA
CCCGCGAGACCATGGAGCATGCGATCAATTTTTCCGAAACCGGACACCTCTGTCTGTCGACGCTGCACGCCAACAATGCC
AACCAGGCGCTCGATCGCATCATCAACCTCTTTCCGGAAGAACGCCGGAGTCAGTTGCTGATGGATCTCTCGCTCAATCT
TAACGCGGTCATCTCGCAACGTCTGCTGCCCTGCACCGACGGCGGACGCATCGCGGCGGTCGAGGTCATGATCAACTCGC
CGCTGATCCAGGACATGATTTTTAAGGGCGATGTCGGCGGCATCAAAAGCATCATGAAAAAGTCCCGCGAACTCGGCATG
CAGACCTTCGATATGTCGCTGTTCGATCTGCATGAAGCGGGCAAGATCAATTACGAGGATGCCTTGCGCAATGCCGATTC
CATGAACGGATTGCGCCTGGCGATCAAATTGGAAGGTCGGGAGGCGCGCGGCAAGAACCACTTCGCCGAGACGGATGGCT
TGACCATGGTCGGCGACGAGGAAGAGGATGTCTTCGACTCGACCCTGTTTGGCGAGGCGCTGCCCGTCTCCCGGATCGAG
GAACCACCCGCGGGTCTCGTCAATCCGGCCGGATCGAAGGCGAGACGATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (119-275)

3D structure

• AlphaFold DB: I3YCQ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.563	87.981	0.524
pilU	Acinetobacter baylyi ADP1	56.034	83.654	0.469
pilU	Vibrio cholerae strain A1552	53.179	83.173	0.442
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.768	82.933	0.363