Detailed information NTG0514061

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: KV188_RS09160
• Genome accession: NZ_CP078508
• Coordinates: 1884841..1885845 (+)
• Length: 334 a.a.
• NCBI ID: WP_005600359.1
• Uniprot ID: A3MZ06
• Organism: Actinobacillus pleuropneumoniae serovar 8 str. 405
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1879841..1890845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KV188_RS09140 (KV188_09140)	potA	1881345..1882460 (+)	1116	WP_005600350.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
KV188_RS09145 (KV188_09145)	potB	1882447..1883313 (+)	867	WP_005603539.1	spermidine/putrescine ABC transporter permease PotB	-
KV188_RS09150 (KV188_09150)	potC	1883313..1884080 (+)	768	WP_039708954.1	spermidine/putrescine ABC transporter permease PotC	-
KV188_RS09155 (KV188_09155)	ruvA	1884217..1884822 (+)	606	WP_005600357.1	Holliday junction branch migration protein RuvA	Machinery gene
KV188_RS09160 (KV188_09160)	ruvB	1884841..1885845 (+)	1005	WP_005600359.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
KV188_RS09165 (KV188_09165)	ubiG	1885902..1886606 (-)	705	WP_039708955.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
KV188_RS09170 (KV188_09170)	gyrA	1886786..1889476 (+)	2691	WP_039708956.1	DNA topoisomerase (ATP-hydrolyzing) subunit A	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 36929.33 Da
• Isoelectric Point: 4.7294

>NTDB_id=514061 KV188_RS09160 WP_005600359.1 1884841..1885845(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVREQMEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCLNLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNG
VITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSRTYAHLGIAKLD

Nucleotide

• Length: 1005 bp

>NTDB_id=514061 KV188_RS09160 WP_005600359.1 1884841..1885845(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGATTGAAGCAGATAGAATAATTAGTGCCTCGCCGAAGCGTGAGGAGGAAGTGATTGATCGTGCGATTCGTCCTAAATT
ATTGGCGGATTATGTCGGTCAGCCGTCTGTACGAGAGCAGATGGAGATCTTTATTAAAGCGGCAAAATTGCGTGATGAGG
CGCTAGATCACTTATTAATTTTCGGCCCTCCGGGATTGGGTAAAACCACCTTAGCTAATATTGTTGCCAACGAAATGGGC
GTGAATATTCGTACCACTTCCGGACCTGTGTTAGAGAAAGCGGGCGATTTGGCGGCAATGCTGACCAATTTAGAACCTTA
TGACGTATTATTTATTGATGAGATCCACCGCTTATCGCCGGCAATTGAAGAAGTGCTTTATCCTGCGATGGAAGATTATC
AGCTGGATATCATGATCGGTGAAGGGCCGGCGGCACGTTCAATTAAACTGGATTTACCGCCGTTTACCTTAGTGGGGGCA
ACCACCCGTGCCGGTTCACTGACTTCTCCGTTACGTGACCGTTTTGGAATTGTACAGCGTTTAGAGTTTTACTCGGTAGA
TGATTTAACCTCGATCGTAAAACGTAGTGCCGATTGCTTAAACTTAAATTTATCGCCGGATGGTGCTTATGAAGTGGCTC
GCCGTTCACGTGGCACCCCTCGAATTGCTAACCGTTTATTACGCCGTGTGCGAGATTATGCCGATGTGCGTAATAACGGC
GTGATTACCTCTGATATTGCTAAACAAGCGTTGGCGATGCTTGATGTAGATTCCGAAGGCTTTGATTTTATGGACATCAA
ATTATTGCAAGCGATTGTCGAGCGTTTTGACGGAGGCCCGGTCGGCTTAGATAATTTAGCTGCAGCGATAGGTGAAGAGC
GAGATACGATTGAAGACGTACTTGAACCTTATTTGATTCAGCAGGGTTTTTTACAGCGAACGCCACGAGGGCGTATTGCT
ACTAGCCGAACTTATGCACATTTAGGCATTGCAAAATTAGATTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

3D structure

• AlphaFold DB: A3MZ06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	62.346	97.006	0.605
ruvB	Streptococcus pneumoniae TIGR4	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae R6	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae D39	59.259	97.006	0.575
ruvB	Synechocystis sp. PCC 6803	54.969	96.407	0.53
ruvB	Helicobacter pylori 26695	54.952	93.713	0.515