Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   I8F49_RS04810 Genome accession   NZ_CP065752
Coordinates   993420..993905 (+) Length   161 a.a.
NCBI ID   WP_214329081.1    Uniprot ID   -
Organism   Enterococcus faecium strain 18-465     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 988420..998905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8F49_RS04770 (I8F49_04750) - 988667..989134 (-) 468 WP_229205984.1 hypothetical protein -
  I8F49_RS04775 (I8F49_04755) - 989272..989613 (+) 342 WP_002333656.1 hypothetical protein -
  I8F49_RS04780 (I8F49_04760) - 989606..990277 (+) 672 WP_002333655.1 DUF1071 domain-containing protein -
  I8F49_RS04785 (I8F49_04765) - 990283..990969 (+) 687 WP_002375505.1 putative HNHc nuclease -
  I8F49_RS12620 - 990972..991670 (+) 699 WP_002346395.1 helix-turn-helix domain-containing protein -
  I8F49_RS04795 (I8F49_04775) - 991660..992898 (+) 1239 WP_002346394.1 DnaB-like helicase C-terminal domain-containing protein -
  I8F49_RS04800 (I8F49_04780) - 992899..993087 (+) 189 WP_002346393.1 hypothetical protein -
  I8F49_RS04805 (I8F49_04785) - 993084..993419 (+) 336 WP_002346392.1 VRR-NUC domain-containing protein -
  I8F49_RS04810 (I8F49_04790) ssbA 993420..993905 (+) 486 WP_214329081.1 single-stranded DNA-binding protein Machinery gene
  I8F49_RS04815 (I8F49_04795) - 994314..994631 (+) 318 WP_214329082.1 VRR-NUC domain-containing protein -
  I8F49_RS04820 (I8F49_04800) - 994628..994789 (+) 162 WP_002323911.1 antitoxin -
  I8F49_RS04825 (I8F49_04805) - 994786..995091 (+) 306 WP_214329083.1 hypothetical protein -
  I8F49_RS04830 (I8F49_04810) - 995091..995405 (+) 315 WP_002335860.1 DUF1140 family protein -
  I8F49_RS04835 (I8F49_04815) - 995582..995893 (+) 312 WP_002330296.1 MazG-like family protein -
  I8F49_RS04840 (I8F49_04820) - 995890..996525 (+) 636 WP_214329084.1 DUF1642 domain-containing protein -
  I8F49_RS04845 (I8F49_04825) - 996522..996821 (+) 300 WP_010721543.1 hypothetical protein -
  I8F49_RS04850 (I8F49_04830) - 996811..996975 (+) 165 WP_002304478.1 hypothetical protein -
  I8F49_RS04855 (I8F49_04835) - 997064..997480 (+) 417 WP_002323917.1 ArpU family phage packaging/lysis transcriptional regulator -
  I8F49_RS12625 - 997770..998000 (+) 231 WP_104775165.1 hypothetical protein -
  I8F49_RS04860 (I8F49_04840) - 997997..998197 (+) 201 WP_002333645.1 hypothetical protein -
  I8F49_RS12855 - 998319..998549 (+) 231 WP_214329085.1 HNH endonuclease signature motif containing protein -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 17839.65 Da        Isoelectric Point: 8.9394

>NTDB_id=513984 I8F49_RS04810 WP_214329081.1 993420..993905(+) (ssbA) [Enterococcus faecium strain 18-465]
MINTVTLVGRLTKDPELRTTTSGTGVATFTLAVNRDFKDANGNREADFINCVAWRKTAEILANYAKKGSLIGLIGRIQTR
SYENQQGNRVFVTEVVVERFNLLESRSNNDTSVSNNKTTNYKGNNQNSSNDLSHAQNNQSKNVNSNPFDGSSMNTDLELP
F

Nucleotide


Download         Length: 486 bp        

>NTDB_id=513984 I8F49_RS04810 WP_214329081.1 993420..993905(+) (ssbA) [Enterococcus faecium strain 18-465]
ATGATTAATACAGTGACTTTAGTAGGAAGATTGACAAAAGACCCAGAGCTTAGAACCACAACAAGTGGAACAGGAGTAGC
GACTTTCACATTAGCAGTAAATCGTGACTTCAAAGATGCGAATGGAAATAGAGAAGCAGACTTTATCAATTGTGTAGCGT
GGAGAAAAACAGCTGAAATTTTAGCAAACTATGCGAAAAAAGGTTCGTTGATTGGATTGATTGGGCGTATTCAAACCAGA
AGCTATGAAAATCAACAAGGAAACCGAGTTTTTGTGACGGAAGTAGTCGTAGAACGCTTCAATCTACTTGAGAGCCGTTC
TAACAACGATACAAGCGTTTCGAACAATAAGACGACAAATTATAAAGGGAACAACCAAAACAGCTCTAACGACTTATCAC
ACGCTCAAAACAATCAATCAAAAAACGTTAATTCCAATCCGTTTGATGGTTCGTCAATGAATACTGATTTAGAACTTCCG
TTTTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

50.581

100

0.54
  ssb Latilactobacillus sakei subsp. sakei 23K

51.176

100

0.54
  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

65.839

0.385
  ssbB/cilA Streptococcus mitis NCTC 12261

54.206

66.46

0.36
  ssbB/cilA Streptococcus pneumoniae TIGR4

54.206

66.46

0.36