Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G32_RS16145 Genome accession   NZ_CP065750
Coordinates   3493225..3494928 (+) Length   567 a.a.
NCBI ID   WP_013981942.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain FDAARGOS_875     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3488225..3499928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G32_RS16130 (I6G32_16130) - 3490178..3490732 (-) 555 WP_080564968.1 REP-associated tyrosine transposase -
  I6G32_RS16135 (I6G32_16135) - 3491985..3492266 (-) 282 WP_197932780.1 hypothetical protein -
  I6G32_RS16140 (I6G32_16140) - 3492436..3492915 (-) 480 WP_041771654.1 pilin -
  I6G32_RS16145 (I6G32_16145) pilB 3493225..3494928 (+) 1704 WP_013981942.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G32_RS16150 (I6G32_16150) pilC 3494930..3496147 (+) 1218 WP_013981943.1 type II secretion system F family protein Machinery gene
  I6G32_RS16155 (I6G32_16155) pilD 3496150..3497019 (+) 870 WP_013981944.1 prepilin peptidase Machinery gene
  I6G32_RS16160 (I6G32_16160) coaE 3497150..3497758 (+) 609 WP_013981945.1 dephospho-CoA kinase -
  I6G32_RS16165 (I6G32_16165) yacG 3497755..3497952 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  I6G32_RS16170 (I6G32_16170) - 3497965..3498645 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  I6G32_RS16175 (I6G32_16175) - 3498702..3499742 (-) 1041 WP_013981947.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61895.94 Da        Isoelectric Point: 5.3572

>NTDB_id=513951 I6G32_RS16145 WP_013981942.1 3493225..3494928(+) (pilB) [Stutzerimonas stutzeri strain FDAARGOS_875]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAVRGKTPFVTYVVQNKLAKGRAIAELASDQFGVALLDLSALNKESLPKD
LISEKLARQHRVLPLSKRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGATGLEELGEVDLD
GVDVESVTEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=513951 I6G32_RS16145 WP_013981942.1 3493225..3494928(+) (pilB) [Stutzerimonas stutzeri strain FDAARGOS_875]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCTCAAGCCGTTCGCGGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCCA
TAGCCGAGCTTGCTTCCGATCAATTCGGTGTAGCGCTGCTGGACCTGTCTGCGCTGAACAAGGAGTCGCTGCCCAAGGAT
CTGATCAGTGAGAAGTTGGCCCGCCAACATCGGGTGCTGCCGCTTTCCAAGCGCGGCACCAAGCTTTATGTCGCCATCTC
CGATCCGGCTAATCATCAATCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATTCTGGTGGAAGACG
ACAAGCTGGGCGAGGCCATCGACAAACTGTACGAAAGCGGCGCCACCGGACTAGAGGAGCTGGGCGAAGTCGATCTGGAT
GGGGTCGATGTCGAGTCCGTCACCGAAGATGGAAAAGCTGAGGAAGGCGGAGAGGCTGCTGACGACGCCCCTGTAGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGTGGCTCGTCCGATTTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTGCGGCTTCGCACGGATGGCATCCTGCATGAGGTCGCGCGTCCGCCGATTCAGCTTGCACCGCGAATTTCTGCGCGC
CTAAAGGTCATGGCCGGGCTGGATATCTCCGAGCGGCGTAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCTAAGAC
CAAGGCCATCGATTTCCGCGTAAATACGCTACCCACCCTATGGGGCGAGAAAATCGTGATGCGTATCCTCGACCCGTCCA
GTGCGCAGATGGGGATCGATGCCCTGGGTTACGAGGAAGAACAGAAAGAGCTCTACATGAATGCCTTGCGCCAGCCACAA
GGCATGATCCTGGTTACCGGCCCGACTGGGTCGGGCAAGACGGTTTCGCTGTATACCGGTCTGAATATCCTCAATACCGT
GGACGTGAACATCTCCACCGCGGAAGACCCAGTGGAGATCAATCTGGAGGGTATTAACCAGGTCAACGTAAACCCGCGCC
AAGGTATGGACTTCGCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATTATGGTGGGCGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGTAATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAATATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCGCGAAAGCTTTGCAATAGCTGCAAGAAAGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAACCTTCAAGATCTATGGTCCGGTTGGTTGCGACAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCGTTGGCGAGGATTATCATGGAGGACGGCAATTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTACTTCGGCCCTAGTGAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.503

99.295

0.591
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

52.308

91.711

0.48
  pilB Vibrio cholerae strain A1552

53.021

90.476

0.48
  pilF Neisseria gonorrhoeae MS11

48.135

99.295

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.266

96.12

0.377