Detailed information NTG0513953

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I6G32_RS16155
• Genome accession: NZ_CP065750
• Coordinates: 3496150..3497019 (+)
• Length: 289 a.a.
• NCBI ID: WP_013981944.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain FDAARGOS_875
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3491150..3502019

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G32_RS16135 (I6G32_16135)	-	3491985..3492266 (-)	282	WP_197932780.1	hypothetical protein	-
I6G32_RS16140 (I6G32_16140)	-	3492436..3492915 (-)	480	WP_041771654.1	pilin	-
I6G32_RS16145 (I6G32_16145)	pilB	3493225..3494928 (+)	1704	WP_013981942.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I6G32_RS16150 (I6G32_16150)	pilC	3494930..3496147 (+)	1218	WP_013981943.1	type II secretion system F family protein	Machinery gene
I6G32_RS16155 (I6G32_16155)	pilD	3496150..3497019 (+)	870	WP_013981944.1	prepilin peptidase	Machinery gene
I6G32_RS16160 (I6G32_16160)	coaE	3497150..3497758 (+)	609	WP_013981945.1	dephospho-CoA kinase	-
I6G32_RS16165 (I6G32_16165)	yacG	3497755..3497952 (+)	198	WP_041771656.1	DNA gyrase inhibitor YacG	-
I6G32_RS16170 (I6G32_16170)	-	3497965..3498645 (-)	681	WP_013981946.1	energy-coupling factor ABC transporter permease	-
I6G32_RS16175 (I6G32_16175)	-	3498702..3499742 (-)	1041	WP_013981947.1	NADP(H)-dependent aldo-keto reductase	-
I6G32_RS16180 (I6G32_16180)	rplM	3500162..3500590 (+)	429	WP_003287302.1	50S ribosomal protein L13	-
I6G32_RS16185 (I6G32_16185)	rpsI	3500606..3500998 (+)	393	WP_013981948.1	30S ribosomal protein S9	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32166.07 Da
• Isoelectric Point: 7.2753

>NTDB_id=513953 I6G32_RS16155 WP_013981944.1 3496150..3497019(+) (pilD) [Stutzerimonas stutzeri strain FDAARGOS_875]
MGLLDYLASHVLAFVLSAAVLGLLVGSFLNVVIYRLPIMMQREWRMQAREYLEYPPEPIGERFNLLVPSSRCPHCGHRIR
AWENIPLVSWLALRGKCSSCQAPISCRYPLVELACGVLSGYVAWHFGFSWQAGAMLLLTWGVLAMSMIDVDHQLLPDSLV
LPLLWLGLIANNFTLFASLEDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVG
AVLGSILLRVQRAESSTPIPFGPYLAIAGWIALLWGDRITESYLQFARF

Nucleotide

• Length: 870 bp

>NTDB_id=513953 I6G32_RS16155 WP_013981944.1 3496150..3497019(+) (pilD) [Stutzerimonas stutzeri strain FDAARGOS_875]
ATGGGGCTGCTCGATTATTTGGCCAGCCACGTGCTGGCCTTTGTTTTGTCTGCTGCAGTGCTCGGTCTGCTGGTTGGCAG
CTTTCTCAATGTCGTGATTTACCGTTTGCCAATAATGATGCAGCGCGAATGGCGCATGCAGGCGCGGGAATATTTGGAGT
ATCCGCCCGAGCCAATTGGGGAGCGTTTCAATCTACTGGTGCCGAGTTCACGCTGCCCTCACTGCGGTCATCGTATCCGT
GCCTGGGAGAACATTCCCTTGGTCAGCTGGCTGGCGCTGCGTGGAAAGTGCTCGTCTTGTCAGGCGCCGATCAGCTGTCG
ATATCCGTTGGTTGAGCTGGCGTGTGGCGTGCTATCCGGATACGTGGCATGGCATTTCGGTTTTTCCTGGCAGGCAGGGG
CGATGCTTCTGCTGACCTGGGGGGTGCTGGCGATGAGCATGATCGATGTCGATCATCAGTTGCTGCCTGACTCGCTCGTG
CTGCCGCTCTTGTGGTTAGGGTTGATTGCCAACAACTTTACTCTGTTCGCCTCTCTCGAGGATGCGCTGTGGGGGGCGAT
AGCCGGTTACCTGAGCCTATGGTCCGTTTATTGGCTGTTCAAGCTGGTCACAGGCAAAGAGGGTATGGGCTACGGCGATT
TCAAGCTGCTGGCGATGCTTGGTGCCTGGGGCGGCTGGCAGGTTCTGCCGCTCACTATCTTGCTGTCTTCGGTGGTCGGC
GCCGTGCTTGGCAGTATTTTGCTGAGGGTGCAGCGGGCTGAAAGCAGTACGCCCATTCCGTTCGGGCCGTATCTGGCGAT
AGCGGGGTGGATTGCGTTGCTTTGGGGCGACCGAATTACCGAAAGCTACCTCCAGTTCGCTCGCTTCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.818	95.156	0.55
pilD	Vibrio campbellii strain DS40M4	57.143	94.464	0.54
pilD	Acinetobacter baumannii D1279779	50.352	98.27	0.495
pilD	Acinetobacter nosocomialis M2	50.352	98.27	0.495
pilD	Neisseria gonorrhoeae MS11	52.852	91.003	0.481