Detailed information NTG0051256

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MUS_RS13335
• Genome accession: NC_017912
• Coordinates: 2744346..2744960 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis YAU B9601-Y2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2739346..2749960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUS_RS13310 (MUS_2850)	rpsT	2739649..2739915 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MUS_RS13315 (MUS_2851)	holA	2739932..2740973 (-)	1042	Protein_2644	DNA polymerase III subunit delta	-
MUS_RS21750	-	2741013..2741164 (-)	152	Protein_2645	hypothetical protein	-
MUS_RS21270 (MUS_2852)	-	2741205..2741339 (+)	135	WP_003152870.1	YqzM family protein	-
MUS_RS13325 (MUS_2853)	comEC	2741358..2743709 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MUS_RS13330 (MUS_2854)	-	2743710..2744279 (-)	570	WP_003152868.1	ComE operon protein 2	-
MUS_RS13335 (MUS_2855)	comEA	2744346..2744960 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
MUS_RS13340 (MUS_2856)	comER	2745019..2745840 (+)	822	WP_014418420.1	late competence protein ComER	-
MUS_RS13345 (MUS_2857)	-	2745909..2746646 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
MUS_RS13350 (MUS_2858)	rsfS	2746643..2746999 (-)	357	WP_007408260.1	ribosome silencing factor	-
MUS_RS13355 (MUS_2859)	yqeK	2747017..2747577 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MUS_RS13360 (MUS_2860)	-	2747567..2748136 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MUS_RS13365 (MUS_2861)	yhbY	2748147..2748437 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MUS_RS13370 (MUS_2862)	aroE	2748431..2749273 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=51256 MUS_RS13335 WP_014418419.1 2744346..2744960(-) (comEA) [Bacillus velezensis YAU B9601-Y2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=51256 MUS_RS13335 WP_014418419.1 2744346..2744960(-) (comEA) [Bacillus velezensis YAU B9601-Y2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363