Detailed information NTG0507770

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KU204_RS14495
• Genome accession: NZ_CP077837
• Coordinates: 3010532..3011650 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain DETAB-E159
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3005532..3016650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU204_RS14485 (KU204_14425)	-	3008648..3009340 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
KU204_RS14490 (KU204_14430)	pilT	3009467..3010504 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KU204_RS14495 (KU204_14435)	pilU	3010532..3011650 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KU204_RS14500 (KU204_14440)	fur	3011720..3012157 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KU204_RS14505 (KU204_14445)	bamE	3012269..3012667 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KU204_RS14510 (KU204_14450)	-	3012722..3013045 (-)	324	WP_000442587.1	RnfH family protein	-
KU204_RS14515 (KU204_14455)	-	3013045..3014112 (-)	1068	WP_002019572.1	hypothetical protein	-
KU204_RS14520 (KU204_14460)	-	3014265..3014720 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KU204_RS14525 (KU204_14465)	-	3014973..3015821 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=507770 KU204_RS14495 WP_000347039.1 3010532..3011650(+) (pilU) [Acinetobacter baumannii strain DETAB-E159]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=507770 KU204_RS14495 WP_000347039.1 3010532..3011650(+) (pilU) [Acinetobacter baumannii strain DETAB-E159]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395