Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IR195_RS10500 Genome accession   NZ_CP065135
Coordinates   2192216..2193928 (+) Length   570 a.a.
NCBI ID   WP_241006830.1    Uniprot ID   -
Organism   Vreelandella venusta strain PBH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2187216..2198928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IR195_RS10470 (IR195_10470) - 2188504..2188863 (+) 360 WP_146943420.1 hypothetical protein -
  IR195_RS10475 (IR195_10475) - 2188942..2189694 (+) 753 WP_125748105.1 VIT1/CCC1 transporter family protein -
  IR195_RS10485 (IR195_10485) - 2190044..2190853 (-) 810 WP_206049708.1 inositol monophosphatase family protein -
  IR195_RS10490 (IR195_10490) - 2190944..2191399 (-) 456 WP_206049709.1 anti-virulence regulator CigR family protein -
  IR195_RS20540 (IR195_10495) - 2191565..2191957 (-) 393 WP_277395566.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IR195_RS10500 (IR195_10500) pilB 2192216..2193928 (+) 1713 WP_241006830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IR195_RS10505 (IR195_10505) - 2193935..2195164 (+) 1230 WP_206049711.1 type II secretion system F family protein -
  IR195_RS10510 (IR195_10510) pilD 2195220..2196065 (+) 846 WP_146943533.1 A24 family peptidase Machinery gene
  IR195_RS10515 (IR195_10515) coaE 2196106..2196702 (+) 597 WP_206049712.1 dephospho-CoA kinase -
  IR195_RS10520 (IR195_10520) yacG 2196742..2196975 (+) 234 WP_083004574.1 DNA gyrase inhibitor YacG -
  IR195_RS10525 (IR195_10525) - 2196989..2197861 (+) 873 WP_206049713.1 DNA replication terminus site-binding protein -
  IR195_RS10530 (IR195_10530) - 2197893..2198234 (-) 342 WP_125748130.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62499.45 Da        Isoelectric Point: 5.5161

>NTDB_id=507161 IR195_RS10500 WP_241006830.1 2192216..2193928(+) (pilB) [Vreelandella venusta strain PBH]
MHSGPHGIAKRLVENGELTDAQATVAENTATELDVSLLQHLVDSGLVESGAAAQAAAWEFGLPVVELDVIRINALPPAND
YPVNLLERLEVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSIDGVLAPVDQLKSTLTQYLAQNERSMLDELDNVDDA
ISALNIVQQDEDEATLDKATKESEDAPVVKFVNKILLDAINRGASDIHFEPYETLYRVRFRIDGMLFEIARPPFALRHRI
AARVKIMSRLDISERRLPQDGAIKLQLSRTHSIDFRVNSLPTVFGEKIVLRLLDPATAQLSIEQLGFSSEQQAVYEHALK
KPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGVNQVNVLPKIGLNFASALRAFLRQDPDVVMVGE
IRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGIASYNIASSVSLIIAQRLARKLCSLCKTPAQIPDEALRQA
GFSQQDIRNGTIYSAVGCKHCTQGYKGRIGIYEVVPVTDAMRQLIMRDVDALNIDQQARREGYPSLYQRCLSRVLDGSTS
LAEVNRISKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=507161 IR195_RS10500 WP_241006830.1 2192216..2193928(+) (pilB) [Vreelandella venusta strain PBH]
ATGCATAGCGGTCCGCACGGTATCGCTAAACGGCTGGTTGAAAACGGCGAGCTGACCGATGCTCAGGCGACTGTCGCGGA
AAACACAGCAACAGAGCTTGATGTGTCGTTGTTACAGCACCTGGTTGATAGCGGGCTAGTAGAATCAGGCGCAGCAGCGC
AGGCCGCTGCCTGGGAGTTTGGCTTGCCGGTCGTTGAGCTAGATGTCATTCGTATAAATGCGCTGCCACCCGCCAACGAC
TATCCCGTTAACTTATTAGAGCGTCTAGAGGTCCTTCCCCTGGCACGCCATGGGCATCGACTGACGGTAGCTGTACCCTA
CCCCGCAACGCTAACCCAGCTAGATGAGCTGCAATTCGCTACCGGGCTAAGTATCGATGGAGTACTGGCTCCCGTTGATC
AACTTAAAAGCACTCTAACGCAGTATCTAGCGCAAAATGAGCGCAGCATGCTAGATGAATTGGACAACGTAGATGATGCG
ATCAGCGCGTTGAATATTGTTCAGCAGGACGAAGATGAGGCAACCTTAGACAAGGCAACAAAAGAGAGTGAAGATGCCCC
CGTCGTCAAATTCGTCAATAAAATACTCTTAGATGCTATCAACCGCGGCGCTTCCGATATTCACTTTGAGCCTTATGAAA
CCCTGTACCGGGTGCGCTTTCGTATTGATGGCATGCTGTTTGAAATTGCCCGCCCACCTTTTGCGCTTCGGCACCGCATT
GCGGCTCGGGTAAAAATCATGTCTCGGCTGGACATCTCTGAGCGGCGCCTACCCCAGGATGGGGCAATCAAGCTGCAGCT
TTCACGCACTCACTCAATTGATTTTCGAGTGAACTCACTGCCCACTGTGTTTGGTGAAAAAATAGTGCTGCGCTTGCTAG
ATCCTGCCACTGCTCAACTCTCTATCGAACAACTAGGCTTTAGCTCTGAACAACAGGCCGTTTATGAACACGCGCTTAAG
AAGCCTCAAGGAATGATTCTGGTTACCGGGCCTACGGGAAGCGGTAAAACAGTGACGCTTTATACAGGCATCAACATTCT
TAACCAAATTGAGCGTAATATTTGCACCGCCGAAGACCCAGTAGAAATTAAAGTGTCGGGGGTCAATCAGGTCAACGTTC
TTCCTAAAATAGGTCTGAACTTCGCTAGTGCTCTACGTGCCTTTTTACGCCAAGATCCCGATGTCGTCATGGTGGGCGAA
ATTCGTGACTTGGAAACCGCCGAAATTGCCGTGAAAGCAGCCCAAACGGGACACTTAGTGCTATCTACCGTACACACAAA
CTCCGCCGCTGAAACACTAACCCGCTTAGCAAATATGGGCATCGCTTCCTATAACATCGCCAGCTCGGTTAGCTTGATCA
TTGCCCAACGCTTGGCACGCAAGCTATGCAGCCTATGCAAAACCCCCGCACAGATTCCTGACGAAGCACTGCGTCAGGCT
GGCTTTAGCCAGCAGGACATTCGCAACGGCACGATTTATTCAGCCGTCGGCTGTAAACACTGCACCCAGGGCTATAAAGG
TCGTATTGGTATCTATGAAGTGGTGCCTGTTACTGATGCCATGCGCCAGTTGATTATGCGTGATGTTGACGCGCTGAATA
TCGATCAGCAAGCACGCCGCGAAGGTTACCCTAGCCTTTATCAACGCTGCTTAAGTCGTGTGCTGGATGGCAGCACTAGC
TTGGCAGAAGTTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-147)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.241

98.947

0.507
  pilB Acinetobacter baumannii D1279779

49.479

100

0.5
  pilB Acinetobacter baylyi ADP1

49.648

99.649

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

48.252

100

0.484
  pilB Vibrio cholerae strain A1552

49.005

97.018

0.475
  pilB Vibrio campbellii strain DS40M4

49.257

94.386

0.465
  pilF Neisseria gonorrhoeae MS11

47.917

84.211

0.404