Detailed information NTG0507162

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IR195_RS10510
• Genome accession: NZ_CP065135
• Coordinates: 2195220..2196065 (+)
• Length: 281 a.a.
• NCBI ID: WP_146943533.1
• Uniprot ID: A0AAP9ZG45
• Organism: Vreelandella venusta strain PBH
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2190220..2201065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IR195_RS10490 (IR195_10490)	-	2190944..2191399 (-)	456	WP_206049709.1	anti-virulence regulator CigR family protein	-
IR195_RS20540 (IR195_10495)	-	2191565..2191957 (-)	393	WP_277395566.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
IR195_RS10500 (IR195_10500)	pilB	2192216..2193928 (+)	1713	WP_241006830.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IR195_RS10505 (IR195_10505)	-	2193935..2195164 (+)	1230	WP_206049711.1	type II secretion system F family protein	-
IR195_RS10510 (IR195_10510)	pilD	2195220..2196065 (+)	846	WP_146943533.1	A24 family peptidase	Machinery gene
IR195_RS10515 (IR195_10515)	coaE	2196106..2196702 (+)	597	WP_206049712.1	dephospho-CoA kinase	-
IR195_RS10520 (IR195_10520)	yacG	2196742..2196975 (+)	234	WP_083004574.1	DNA gyrase inhibitor YacG	-
IR195_RS10525 (IR195_10525)	-	2196989..2197861 (+)	873	WP_206049713.1	DNA replication terminus site-binding protein	-
IR195_RS10530 (IR195_10530)	-	2197893..2198234 (-)	342	WP_125748130.1	hypothetical protein	-
IR195_RS10535 (IR195_10535)	-	2198605..2199438 (+)	834	WP_125748133.1	helix-turn-helix transcriptional regulator	-
IR195_RS10540 (IR195_10540)	-	2199517..2199891 (-)	375	WP_206049714.1	histone-like nucleoid-structuring protein, MvaT/MvaU family	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 30914.21 Da
• Isoelectric Point: 8.4767

>NTDB_id=507162 IR195_RS10510 WP_146943533.1 2195220..2196065(+) (pilD) [Vreelandella venusta strain PBH]
MGLPSNCYLFFALLIGLCFGSFLNVVITRLPVMLMRRWRNEARVSLELVEETSPRFNLATPRSMCPRCEQPIAWHDNLPL
IGWLKRRGKCAHCQTSISVQYPFVELMGGLLAVVVVALHGPTLNAMFIYGACVTLLALAVIDFRTYLLPDMLTLPLLWAG
LVYQLLFQPQLLSGAVIGAMAGYLVLWAFYWAFKLITGKEGMGFGDFKLLAALGAWVGWSMLPLLLILSAGIGAAIGLAA
QALFPQLRGQPLPFGPFLALAGWICILVGNELMALYTSLLY

Nucleotide

• Length: 846 bp

>NTDB_id=507162 IR195_RS10510 WP_146943533.1 2195220..2196065(+) (pilD) [Vreelandella venusta strain PBH]
ATGGGTTTACCGTCAAACTGTTACCTCTTTTTTGCTTTACTAATCGGCCTCTGCTTTGGCAGTTTTCTTAACGTTGTCAT
CACTCGTTTACCCGTTATGCTAATGCGCCGTTGGCGTAATGAAGCACGCGTCTCTCTTGAGCTGGTCGAAGAAACATCCC
CCCGCTTTAACCTTGCTACGCCAAGGTCAATGTGTCCACGCTGCGAACAACCCATTGCCTGGCACGATAACCTACCTTTA
ATAGGCTGGCTAAAAAGAAGGGGAAAATGCGCCCATTGCCAAACCTCCATCAGTGTACAGTACCCCTTCGTTGAGCTGAT
GGGCGGACTACTTGCAGTAGTCGTTGTGGCCTTGCATGGCCCAACGCTAAACGCAATGTTTATCTATGGCGCTTGTGTCA
CGCTACTGGCCTTGGCAGTAATCGACTTTCGTACCTATTTACTCCCTGATATGTTAACCCTTCCGCTATTATGGGCTGGC
TTAGTTTACCAATTACTCTTTCAGCCACAGCTGCTTTCTGGCGCTGTCATCGGCGCAATGGCGGGCTATCTTGTGCTATG
GGCTTTCTACTGGGCTTTTAAGCTCATAACAGGTAAAGAAGGCATGGGGTTCGGTGATTTCAAATTACTCGCTGCCCTTG
GCGCTTGGGTAGGTTGGAGCATGTTACCGTTGCTGCTTATTTTATCTGCGGGAATTGGCGCAGCCATAGGATTAGCCGCT
CAAGCTCTATTTCCTCAACTGCGCGGCCAACCTCTCCCCTTCGGTCCCTTTCTTGCGCTGGCGGGCTGGATTTGCATACT
GGTAGGCAACGAGTTAATGGCGCTCTATACGTCGTTGCTGTATTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-116)

• Type IV leader peptidase family (Peptidase_A24) (132-238)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.545	97.865	0.495
pilD	Acinetobacter baumannii D1279779	47.015	95.374	0.448
pilD	Acinetobacter nosocomialis M2	47.015	95.374	0.448
pilD	Vibrio campbellii strain DS40M4	44.404	98.577	0.438
pilD	Neisseria gonorrhoeae MS11	44.906	94.306	0.423