Detailed information NTG0050623

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MY9_RS12900
• Genome accession: NC_017743
• Coordinates: 2546283..2546900 (-)
• Length: 205 a.a.
• NCBI ID: WP_014664669.1
• Uniprot ID: -
• Organism: Bacillus sp. JS
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2541283..2551900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MY9_RS12875 (MY9_2580)	rpsT	2541584..2541850 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
MY9_RS12880 (MY9_2581)	holA	2541865..2542908 (-)	1044	WP_014664666.1	DNA polymerase III subunit delta	-
MY9_RS21610	-	2542948..2543061 (-)	114	WP_134975484.1	hypothetical protein	-
MY9_RS12885 (MY9_2582)	-	2543138..2543272 (+)	135	WP_003229983.1	YqzM family protein	-
MY9_RS12890 (MY9_2583)	comEC	2543313..2545643 (-)	2331	WP_014664667.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MY9_RS12895 (MY9_2584)	-	2545647..2546216 (-)	570	WP_014664668.1	ComE operon protein 2	-
MY9_RS12900 (MY9_2585)	comEA	2546283..2546900 (-)	618	WP_014664669.1	helix-hairpin-helix domain-containing protein	Machinery gene
MY9_RS12905 (MY9_2586)	comER	2546984..2547805 (+)	822	WP_004398597.1	late competence protein ComER	-
MY9_RS12910 (MY9_2587)	-	2547871..2548614 (-)	744	WP_014664670.1	class I SAM-dependent methyltransferase	-
MY9_RS12915 (MY9_2588)	rsfS	2548611..2548967 (-)	357	WP_014664671.1	ribosome silencing factor	-
MY9_RS12920 (MY9_2589)	yqeK	2548985..2549545 (-)	561	WP_014664672.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MY9_RS12925 (MY9_2590)	-	2549535..2550104 (-)	570	WP_014664673.1	nicotinate-nucleotide adenylyltransferase	-
MY9_RS12930 (MY9_2591)	yhbY	2550116..2550406 (-)	291	WP_014664674.1	ribosome assembly RNA-binding protein YhbY	-
MY9_RS12935 (MY9_2592)	aroE	2550400..2551242 (-)	843	WP_014664675.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21759.57 Da
• Isoelectric Point: 4.9757

>NTDB_id=50623 MY9_RS12900 WP_014664669.1 2546283..2546900(-) (comEA) [Bacillus sp. JS]
MNWLNQHKKAMILAASAAVFTAIMIFLASGRNKEPVKQAVPTETVNTEVKQEAGKDEPSEAIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTNEQADEVQVNLAEILQDGTVVYIPKKGEETAVQQGAGGAVQSGGGKGELVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFHTIEDITKVSGIGEKSFEKIKSSISVK

Nucleotide

• Length: 618 bp

>NTDB_id=50623 MY9_RS12900 WP_014664669.1 2546283..2546900(-) (comEA) [Bacillus sp. JS]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATGATTTTAGCGGCTTCTGCAGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCAGCGGGAGAAACAAAGAGCCGGTGAAGCAGGCTGTACCAACTGAGACGGTAAATACAGAGGTAAAGCAGGAAGCAG
GCAAAGATGAGCCAAGCGAAGCAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGTGGAACCAATGAGCAAGCTGATGAAGTGCAAGTGAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAAGGAGAGGAAACAGCAGTGCAGCAGGGTGCCGGAGGAGCTG
TTCAAAGCGGTGGAGGGAAGGGGGAGCTGGTAAATATCAATACAGCCACCTTAGAGGAGTTACAAGGCATCTCAGGAGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCATACTATTGAAGATATCACGAAGGT
TTCAGGAATAGGCGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTTCAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	91.22	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	35.398	100	0.39
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.579	92.683	0.385
comEA/celA/cilE	Streptococcus pneumoniae D39	41.579	92.683	0.385
comEA/celA/cilE	Streptococcus pneumoniae R6	41.579	92.683	0.385