Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   KQ217_RS10820 Genome accession   NZ_CP076706
Coordinates   2215723..2216847 (-) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0H2VB93
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2210723..2221847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS10790 (KQ217_10790) yrdA 2212523..2213077 (+) 555 WP_001286206.1 gamma carbonic anhydrase family protein -
  KQ217_RS10795 (KQ217_10795) yrdB 2213053..2213310 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  KQ217_RS10800 (KQ217_10800) aroE 2213307..2214125 (-) 819 WP_001683342.1 shikimate dehydrogenase -
  KQ217_RS10805 (KQ217_10805) tsaC 2214130..2214702 (-) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  KQ217_RS10810 (KQ217_10810) yrdD 2214707..2215249 (-) 543 WP_001312137.1 type I DNA topoisomerase -
  KQ217_RS10815 (KQ217_10815) smg 2215278..2215751 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  KQ217_RS10820 (KQ217_10820) dprA 2215723..2216847 (-) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  KQ217_RS10825 (KQ217_10825) def 2216977..2217486 (+) 510 WP_000114984.1 peptide deformylase -
  KQ217_RS10830 (KQ217_10830) fmt 2217501..2218448 (+) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  KQ217_RS10835 (KQ217_10835) rsmB 2218500..2219789 (+) 1290 WP_000744767.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  KQ217_RS10840 (KQ217_10840) trkA 2219811..2221187 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  KQ217_RS10845 (KQ217_10845) mscL 2221317..2221730 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=504223 KQ217_RS10820 WP_000228508.1 2215723..2216847(-) (dprA) [Escherichia coli O170:H18 strain CFIAFB20120266]
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=504223 KQ217_RS10820 WP_000228508.1 2215723..2216847(-) (dprA) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VB93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39