Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KQ217_RS09160 Genome accession   NZ_CP076706
Coordinates   1870426..1871919 (-) Length   497 a.a.
NCBI ID   WP_097697392.1    Uniprot ID   -
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1865426..1876919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS09130 (KQ217_09130) gspK 1866220..1867197 (-) 978 WP_000633183.1 type II secretion system minor pseudopilin GspK -
  KQ217_RS09135 (KQ217_09135) gspJ 1867194..1867799 (-) 606 WP_216803833.1 type II secretion system minor pseudopilin GspJ -
  KQ217_RS09140 (KQ217_09140) gspI 1867796..1868167 (-) 372 WP_000820120.1 type II secretion system minor pseudopilin GspI -
  KQ217_RS09145 (KQ217_09145) gspH 1868164..1868727 (-) 564 WP_001115124.1 type II secretion system minor pseudopilin GspH -
  KQ217_RS09150 (KQ217_09150) gspG 1868731..1869186 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  KQ217_RS09155 (KQ217_09155) gspF 1869203..1870426 (-) 1224 WP_001173408.1 type II secretion system inner membrane protein GspF -
  KQ217_RS09160 (KQ217_09160) pilB/pilB1 1870426..1871919 (-) 1494 WP_097697392.1 type II secretion system ATPase GspE Machinery gene
  KQ217_RS09165 (KQ217_09165) gspD 1871919..1873979 (-) 2061 WP_001314154.1 type II secretion system secretin GspD -
  KQ217_RS09170 (KQ217_09170) gspC 1874009..1874968 (-) 960 WP_042074603.1 type II secretion system protein GspC -
  KQ217_RS09175 (KQ217_09175) gspS2 1874986..1875396 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  KQ217_RS09180 (KQ217_09180) pilD 1875462..1876271 (-) 810 WP_001334345.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55161.35 Da        Isoelectric Point: 5.8815

>NTDB_id=504217 KQ217_RS09160 WP_097697392.1 1870426..1871919(-) (pilB/pilB1) [Escherichia coli O170:H18 strain CFIAFB20120266]
MVPVAQEITANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRMLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=504217 KQ217_RS09160 WP_097697392.1 1870426..1871919(-) (pilB/pilB1) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGGTGCCTGTAGCACAGGAAATCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGATGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCATGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362