Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KQ217_RS09180 Genome accession   NZ_CP076706
Coordinates   1875462..1876271 (-) Length   269 a.a.
NCBI ID   WP_001334345.1    Uniprot ID   -
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1870462..1881271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS09165 (KQ217_09165) gspD 1871919..1873979 (-) 2061 WP_001314154.1 type II secretion system secretin GspD -
  KQ217_RS09170 (KQ217_09170) gspC 1874009..1874968 (-) 960 WP_042074603.1 type II secretion system protein GspC -
  KQ217_RS09175 (KQ217_09175) gspS2 1874986..1875396 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  KQ217_RS09180 (KQ217_09180) pilD 1875462..1876271 (-) 810 WP_001334345.1 prepilin peptidase PppA Machinery gene
  KQ217_RS09185 (KQ217_09185) sslE 1876466..1881028 (-) 4563 WP_216803834.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29429.08 Da        Isoelectric Point: 8.3801

>NTDB_id=504218 KQ217_RS09180 WP_001334345.1 1875462..1876271(-) (pilD) [Escherichia coli O170:H18 strain CFIAFB20120266]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=504218 KQ217_RS09180 WP_001334345.1 1875462..1876271(-) (pilD) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGTCCGCATTGTCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCTTGGCTGATGCTCAAAGGGCGCTGCCGTGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAATT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CGTGGCTGATTGCCGCGAGCATCATCGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGTTAACGCTACAAGACGCAGTCACCGGCGTTCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTGGCTTTAATCGCATCATGCTGCGGTCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375