Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IUJ45_RS07950 Genome accession   NZ_CP064367
Coordinates   1558180..1559856 (+) Length   558 a.a.
NCBI ID   WP_082308679.1    Uniprot ID   -
Organism   Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1553180..1564856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ45_RS07910 (IUJ45_08035) rpmB 1553270..1553503 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  IUJ45_RS07915 (IUJ45_08040) rpmG 1553535..1553690 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  IUJ45_RS07920 (IUJ45_08045) ubiM 1553961..1555145 (-) 1185 WP_002239495.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  IUJ45_RS07925 (IUJ45_08050) rpmA 1555484..1555756 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  IUJ45_RS07930 (IUJ45_08055) rplU 1555781..1556089 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  IUJ45_RS07935 (IUJ45_08065) ispB 1556313..1557287 (+) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  IUJ45_RS07940 (IUJ45_08070) - 1557292..1557738 (+) 447 WP_002233503.1 DUF441 domain-containing protein -
  IUJ45_RS07950 (IUJ45_08080) pilF 1558180..1559856 (+) 1677 WP_082308679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IUJ45_RS07955 (IUJ45_08085) - 1559941..1560150 (-) 210 WP_002249247.1 DNA gyrase inhibitor YacG -
  IUJ45_RS07960 (IUJ45_08090) coaE 1560143..1560775 (-) 633 WP_002226983.1 dephospho-CoA kinase -
  IUJ45_RS07965 (IUJ45_08095) pilD 1560777..1561637 (-) 861 WP_002226036.1 A24 family peptidase Machinery gene
  IUJ45_RS07970 (IUJ45_08100) pilG 1561711..1562943 (-) 1233 WP_002248468.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61993.10 Da        Isoelectric Point: 5.4180

>NTDB_id=501184 IUJ45_RS07950 WP_082308679.1 1558180..1559856(+) (pilF) [Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGRLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=501184 IUJ45_RS07950 WP_082308679.1 1558180..1559856(+) (pilF) [Neisseria meningitidis strain PartJ-Nmeningitidis-RM8376]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCGGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.441

100

0.513
  pilB Acinetobacter baylyi ADP1

50.534

100

0.509
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.014

100

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

93.728

0.373
  pilF Thermus thermophilus HB27

36.949

97.491

0.36