NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	IRJ21_RS04925	Genome accession	NZ_CP064091
Coordinates	1092529..1093143 (-)	Length	204 a.a.
NCBI ID	WP_014418419.1	Uniprot ID	I2C7Y9
Organism	Bacillus velezensis strain C1
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1087529..1098143

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ21_RS04895	rpsT	1087832..1088098 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
IRJ21_RS04900	holA	1088061..1089156 (-)	1096	Protein_978	DNA polymerase III subunit delta	-
IRJ21_RS04905	-	1089196..1089347 (-)	152	Protein_979	hypothetical protein	-
IRJ21_RS04910	-	1089388..1089522 (+)	135	WP_003152870.1	YqzM family protein	-
IRJ21_RS04915	comEC	1089541..1091892 (-)	2352	WP_182071847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IRJ21_RS04920	-	1091893..1092462 (-)	570	WP_003152868.1	ComE operon protein 2	-
IRJ21_RS04925	comEA	1092529..1093143 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
IRJ21_RS04930	comER	1093202..1094023 (+)	822	WP_012118027.1	late competence protein ComER	-
IRJ21_RS04935	-	1094092..1094829 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
IRJ21_RS04940	rsfS	1094826..1095182 (-)	357	WP_007408260.1	ribosome silencing factor	-
IRJ21_RS04945	yqeK	1095200..1095760 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IRJ21_RS04950	-	1095750..1096319 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
IRJ21_RS04955	yhbY	1096330..1096620 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IRJ21_RS04960	aroE	1096614..1097456 (-)	843	WP_182071849.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22226.26 Da Isoelectric Point: 5.0355

                                >NTDB_id=499053 IRJ21_RS04925 WP_014418419.1 1092529..1093143(-) (comEA) [Bacillus velezensis strain C1]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=499053 IRJ21_RS04925 WP_014418419.1 1092529..1093143(-) (comEA) [Bacillus velezensis strain C1]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	I2C7Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363

Detailed information NTG0499053