Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H9K48_RS01605 Genome accession   NZ_CP064033
Coordinates   336858..337451 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain DLM1805     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331858..342451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K48_RS01590 (H9K48_01585) - 332574..335144 (-) 2571 WP_005480476.1 PBP1A family penicillin-binding protein -
  H9K48_RS01595 (H9K48_01590) pilM 335287..336303 (+) 1017 WP_025793368.1 type IV pilus assembly protein PilM Machinery gene
  H9K48_RS01600 (H9K48_01595) pilN 336287..336865 (+) 579 WP_248387347.1 PilN domain-containing protein Machinery gene
  H9K48_RS01605 (H9K48_01600) pilO 336858..337451 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9K48_RS01610 (H9K48_01605) pilP 337441..337956 (+) 516 WP_023585576.1 pilus assembly protein PilP Machinery gene
  H9K48_RS01615 (H9K48_01610) pilQ 337988..339736 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9K48_RS01620 (H9K48_01615) aroK 339925..340443 (+) 519 WP_005381319.1 shikimate kinase AroK -
  H9K48_RS01625 (H9K48_01620) aroB 340475..341575 (+) 1101 WP_025499840.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=498542 H9K48_RS01605 WP_005480482.1 336858..337451(+) (pilO) [Vibrio parahaemolyticus strain DLM1805]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=498542 H9K48_RS01605 WP_005480482.1 336858..337451(+) (pilO) [Vibrio parahaemolyticus strain DLM1805]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGCATCATC
ACCTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726