Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H9K48_RS01595 Genome accession   NZ_CP064033
Coordinates   335287..336303 (+) Length   338 a.a.
NCBI ID   WP_025793368.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain DLM1805     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330287..341303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K48_RS01580 (H9K48_01575) - 330711..331439 (-) 729 WP_015297397.1 redoxin family protein -
  H9K48_RS01585 (H9K48_01580) oxyR 331610..332518 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  H9K48_RS01590 (H9K48_01585) - 332574..335144 (-) 2571 WP_005480476.1 PBP1A family penicillin-binding protein -
  H9K48_RS01595 (H9K48_01590) pilM 335287..336303 (+) 1017 WP_025793368.1 type IV pilus assembly protein PilM Machinery gene
  H9K48_RS01600 (H9K48_01595) pilN 336287..336865 (+) 579 WP_248387347.1 PilN domain-containing protein Machinery gene
  H9K48_RS01605 (H9K48_01600) pilO 336858..337451 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9K48_RS01610 (H9K48_01605) pilP 337441..337956 (+) 516 WP_023585576.1 pilus assembly protein PilP Machinery gene
  H9K48_RS01615 (H9K48_01610) pilQ 337988..339736 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9K48_RS01620 (H9K48_01615) aroK 339925..340443 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37780.32 Da        Isoelectric Point: 6.8323

>NTDB_id=498540 H9K48_RS01595 WP_025793368.1 335287..336303(+) (pilM) [Vibrio parahaemolyticus strain DLM1805]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMMAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=498540 H9K48_RS01595 WP_025793368.1 335287..336303(+) (pilM) [Vibrio parahaemolyticus strain DLM1805]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGATGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ATCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479