Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   KIM25_RS02365 Genome accession   NZ_CP074856
Coordinates   491908..493032 (+) Length   374 a.a.
NCBI ID   WP_000228529.1    Uniprot ID   -
Organism   Escherichia coli strain 2013C_3997_C1     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 486908..498032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM25_RS02340 (KIM25_02340) mscL 487034..487444 (-) 411 WP_000022439.1 large-conductance mechanosensitive channel protein MscL -
  KIM25_RS02345 (KIM25_02345) trkA 487574..488950 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  KIM25_RS02350 (KIM25_02350) rsmB 488972..490261 (-) 1290 WP_032345254.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  KIM25_RS02355 (KIM25_02355) fmt 490307..491254 (-) 948 WP_032345255.1 methionyl-tRNA formyltransferase -
  KIM25_RS02360 (KIM25_02360) def 491269..491778 (-) 510 WP_000114986.1 peptide deformylase -
  KIM25_RS02365 (KIM25_02365) dprA 491908..493032 (+) 1125 WP_000228529.1 DNA-protecting protein DprA Machinery gene
  KIM25_RS02370 (KIM25_02370) smg 493004..493477 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  KIM25_RS02375 (KIM25_02375) yrdD 493506..494048 (+) 543 WP_001129719.1 type I DNA topoisomerase -
  KIM25_RS02380 (KIM25_02380) tsaC 494053..494625 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  KIM25_RS02385 (KIM25_02385) aroE 494630..495448 (+) 819 WP_000451211.1 shikimate dehydrogenase -
  KIM25_RS02390 (KIM25_02390) yrdB 495445..495702 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  KIM25_RS02395 (KIM25_02395) yrdA 495678..496232 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40926.86 Da        Isoelectric Point: 6.3122

>NTDB_id=493838 KIM25_RS02365 WP_000228529.1 491908..493032(+) (dprA) [Escherichia coli strain 2013C_3997_C1]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=493838 KIM25_RS02365 WP_000228529.1 491908..493032(+) (dprA) [Escherichia coli strain 2013C_3997_C1]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393