Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP94_RS10675 Genome accession   NZ_CP063120
Coordinates   2160777..2161319 (-) Length   180 a.a.
NCBI ID   WP_197543617.1    Uniprot ID   A0A7M1NWI5
Organism   Haemophilus parainfluenzae strain M1C137_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2155777..2166319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP94_RS10650 (INP94_10645) - 2156139..2156582 (-) 444 WP_197543613.1 methyltransferase family protein -
  INP94_RS10655 (INP94_10650) - 2156634..2157248 (-) 615 WP_197543614.1 YfgM family protein -
  INP94_RS10660 (INP94_10655) hisS 2157267..2158538 (-) 1272 WP_197543615.1 histidine--tRNA ligase -
  INP94_RS10665 (INP94_10660) ispG 2158548..2159654 (-) 1107 WP_065243889.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP94_RS10670 (INP94_10665) - 2159673..2160701 (-) 1029 WP_197543616.1 RodZ domain-containing protein -
  INP94_RS10675 (INP94_10670) pilF/pilF2 2160777..2161319 (-) 543 WP_197543617.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP94_RS10680 (INP94_10675) - 2161389..2162537 (-) 1149 WP_049363743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP94_RS10685 (INP94_10680) - 2162565..2162834 (+) 270 WP_197544308.1 hypothetical protein -
  INP94_RS10690 (INP94_10685) - 2162840..2163715 (+) 876 WP_197543618.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP94_RS10695 (INP94_10690) - 2163869..2164750 (+) 882 WP_111387504.1 metal ABC transporter substrate-binding protein -
  INP94_RS10700 (INP94_10695) - 2164750..2165646 (+) 897 WP_197543619.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20388.97 Da        Isoelectric Point: 5.9880

>NTDB_id=493090 INP94_RS10675 WP_197543617.1 2160777..2161319(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C137_2]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQSIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLHTLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493090 INP94_RS10675 WP_197543617.1 2160777..2161319(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C137_2]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGCGTTTCTCAATCTTCAGT
CGATTTTAATAAACAACAAGCAGCCAAAGCGCGCGTTGAGTTGGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGAACGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGAGATACGGAAAAAGCGAAGCAAGCTTATTTACAGTCGATTAAATTAGACGATAAGCAAGGTGATGTGTATAA
CAACTTTGGTGCATTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTTGCAGCACCGA
ATTATTATCATCAAGCTGATACTTACGAAAACATAGCACTTTGTGCTTTTGCTGGAAAACAAACCGACGTTTATCAACAA
GCGTTGGATAAATTACGCCAAGTTGATCCTTCTAGAGCAGAAAAGCTCCACACGCTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1NWI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

48.876

98.889

0.483