Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H4O27_RS01875 Genome accession   NZ_CP062976
Coordinates   347081..348760 (+) Length   559 a.a.
NCBI ID   WP_165006782.1    Uniprot ID   -
Organism   Neisseria yangbaofengii strain ZJ785     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342081..353760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4O27_RS01845 - 342119..343057 (-) 939 WP_165006799.1 alpha/beta hydrolase -
  H4O27_RS01850 groES 343393..343680 (+) 288 WP_165006795.1 co-chaperone GroES -
  H4O27_RS01855 - 343728..344018 (+) 291 WP_165006793.1 DUF1330 domain-containing protein -
  H4O27_RS01860 groL 344095..345729 (+) 1635 WP_165006788.1 chaperonin GroEL -
  H4O27_RS01865 - 345852..346418 (-) 567 WP_165006785.1 hypothetical protein -
  H4O27_RS01875 pilF 347081..348760 (+) 1680 WP_165006782.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H4O27_RS01880 pilG 348835..350076 (+) 1242 WP_165006779.1 type II secretion system F family protein Machinery gene
  H4O27_RS01885 pilD 350078..350941 (+) 864 WP_165006776.1 A24 family peptidase Machinery gene
  H4O27_RS01890 coaE 350942..351550 (+) 609 WP_165006774.1 dephospho-CoA kinase -
  H4O27_RS01895 yacG 351553..351738 (+) 186 WP_165006771.1 DNA gyrase inhibitor YacG -
  H4O27_RS01900 ispH 351925..352893 (-) 969 WP_165006768.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  H4O27_RS01905 lspA 352918..353418 (-) 501 WP_165006765.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62327.78 Da        Isoelectric Point: 6.4471

>NTDB_id=492167 H4O27_RS01875 WP_165006782.1 347081..348760(+) (pilF) [Neisseria yangbaofengii strain ZJ785]
MSVGLLRVLAQSQTITPTQAEHYQAAIKAHKDIMPMLFDDGVISPKALGELVARVFSYPLLDLHHYPRSYVLTDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQNFQKIAFSSGVSVDLVVVPHDQLSSLLEWIGQRSTTILKEISQEQESSQPPQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKKPIERPPVPALKKAGFTDEDLAKDWQM
YRPVGCDSCRGKGFKGRIGVYEVMPITEEMQRVIMDNGTEVDIQNMAYQEGMVDLRRAGLLKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=492167 H4O27_RS01875 WP_165006782.1 347081..348760(+) (pilF) [Neisseria yangbaofengii strain ZJ785]
ATGAGTGTCGGATTACTGCGCGTATTGGCGCAAAGTCAAACCATTACGCCTACTCAGGCAGAACATTATCAAGCGGCGAT
TAAAGCGCATAAAGACATTATGCCGATGCTGTTTGATGATGGCGTAATTTCTCCCAAAGCCCTTGGTGAGCTGGTGGCGC
GTGTGTTCAGCTATCCTTTGCTGGATTTGCATCATTATCCGCGCAGCTATGTGTTGACTGATGTGCTGACTGAAGAGCAG
ATGGTGCAAAACCGCTGTGTACCGATTTTCCGTCGTGGCCGTAAGGTTTATTTGGCGGTGTCTGATCCGACCCAAATTCA
AAATTTTCAAAAAATCGCTTTTTCTTCGGGCGTTTCTGTCGATTTGGTGGTAGTGCCGCATGACCAGTTGAGTTCTTTGC
TGGAATGGATTGGCCAACGCTCGACCACTATTTTGAAAGAAATCAGCCAAGAGCAGGAATCTTCGCAACCGCCGCAAGCA
CTGTATATCGACAATGAAGAAGCGGAAGACGGCCCGATTCCGCGCTTCATTCATAAAACATTGTCCGATGCCTTGAATGC
AGGTGCATCGGATATCCATTTTGAATTTTACGAGCAAATGGCACGTGTGCGTTTCCGTGTCGACGGACAATTGCGTGAAG
TAGTGCAGCCGCCGGTTGCCGTGCGCGGTCAGTTGGCATCGCGTATTAAAGTGATGGCGCGCTTGGATATTTCTGAAAAA
CGCGTGCCGCAAGACGGCCGTATTCAGATTGCCTTCCATAAGCATGGCCGCCCGATTGACTTCCGTGTGAGTACCTTGCC
GACTTTGTTCGGTGAAAAAGTGGTGATGCGTATTTTGAATTCGGATGCCGCAACATTGAATATTGACCAATTGGGTTTTG
AGCCATTCCAAAAAGAAATGCTGCTTGAAGCCATTCATCGTCCTTACGGCATGGTGCTGGTGACCGGCCCTACCGGTTCG
GGTAAAACCGTTTCGCTTTATACCTGCCTGAATATTTTGAACACGGAAGATGTGAATATTTCCACGGCAGAAGATCCGGC
CGAGATTAACTTGCCGGGGATTAATCAGGTCAACGTCAATGATAAACAAGGCCTGACCTTTGCCGCGGCCTTGAAATCTT
TCTTGCGTCAGGATCCGGACATCATCATGGTCGGCGAGATTCGTGACTTGGAAACTGCCGACATCGCGATTAAAGCAGCT
CAAACCGGTCATATGGTGTTCTCAACACTGCACACCAATAATGCGCCGGCAACCTTGTCGCGTATGCTGAATATGGGTGT
GGCGCCGTTTAACATAGCCAGTTCGGTAAGTTTGATTATGGCGCAGCGTCTTTTGCGCCGCTTGTGTCCCAACTGTAAAA
AACCGATTGAGCGCCCGCCTGTGCCGGCATTGAAAAAGGCCGGTTTTACCGATGAAGATTTGGCCAAGGATTGGCAAATG
TATCGTCCCGTCGGTTGCGACAGTTGCCGAGGCAAGGGGTTCAAAGGGCGCATCGGTGTGTATGAAGTGATGCCGATTAC
CGAAGAAATGCAGCGTGTGATTATGGATAACGGCACGGAAGTCGATATCCAAAACATGGCTTACCAAGAAGGTATGGTGG
ATTTGCGTCGCGCCGGTTTATTGAAAGTGATGCAGGGTTTGACTTCATTGGAAGAAGTCACGGCACACACCAATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.891

100

0.789
  pilB Acinetobacter baylyi ADP1

51.049

100

0.522
  pilB Acinetobacter baumannii D1279779

51.246

100

0.515
  pilB Legionella pneumophila strain ERS1305867

46.831

100

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

100

0.462
  pilB Vibrio cholerae strain A1552

45.196

100

0.454
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.74

93.739

0.363