Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KFU85_RS03890 Genome accession   NZ_CP074052
Coordinates   822212..823705 (+) Length   497 a.a.
NCBI ID   WP_000249373.1    Uniprot ID   -
Organism   Escherichia coli strain PD23     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817212..828705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU85_RS03870 (KFU85_03830) pilD 817860..818669 (+) 810 WP_001300405.1 prepilin peptidase PppA Machinery gene
  KFU85_RS03875 (KFU85_03835) gspS2 818735..819145 (+) 411 WP_001300497.1 type II secretion system pilot lipoprotein GspS-beta -
  KFU85_RS03880 (KFU85_03840) gspC 819163..820122 (+) 960 WP_135154742.1 type II secretion system protein GspC -
  KFU85_RS03885 (KFU85_03845) gspD 820152..822212 (+) 2061 WP_000498848.1 type II secretion system secretin GspD -
  KFU85_RS03890 (KFU85_03850) pilB/pilB1 822212..823705 (+) 1494 WP_000249373.1 type II secretion system ATPase GspE Machinery gene
  KFU85_RS03895 (KFU85_03855) gspF 823705..824928 (+) 1224 WP_001173446.1 type II secretion system inner membrane protein GspF -
  KFU85_RS03900 (KFU85_03860) gspG 824945..825400 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  KFU85_RS03905 (KFU85_03865) gspH 825404..825967 (+) 564 WP_001115117.1 type II secretion system minor pseudopilin GspH -
  KFU85_RS03910 (KFU85_03870) gspI 825964..826335 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  KFU85_RS03915 (KFU85_03875) gspJ 826332..826937 (+) 606 WP_001332996.1 type II secretion system minor pseudopilin GspJ -
  KFU85_RS03920 (KFU85_03880) gspK 826934..827911 (+) 978 WP_000633203.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55194.27 Da        Isoelectric Point: 6.0089

>NTDB_id=490503 KFU85_RS03890 WP_000249373.1 822212..823705(+) (pilB/pilB1) [Escherichia coli strain PD23]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=490503 KFU85_RS03890 WP_000249373.1 822212..823705(+) (pilB/pilB1) [Escherichia coli strain PD23]
ATGGTGCCTGTAGCACAGGAAACTACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGTTGATGGAAGATCTCGGCTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGTGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362